Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors
| Autor: | Anna Kędziora, Kamila Korzekwa, Dorota Wojnicz, Bartlomiej Stanczykiewicz, Gabriela Bugla-Płoskońska |
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| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
SiO2/Ag0 Organic Chemistry multidrug-resistant bacteria General Medicine biofilm Catalysis Computer Science Applications Pseudomonas aeruginosa Inorganic Chemistry TiO2/Ag0 Chemistry nanoparticles MBIC Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences, Vol 23, Iss 284, p 284 (2022) International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 284 |
| ISSN: | 1422-0067 |
| Popis: | The aim of this study was to assess the beneficial inhibitory effect of silver nanoparticles immobilized on SiO2 or TiO2 on biofilm formation by Pseudomonas aeruginosa—one of the most dangerous pathogens isolated from urine and bronchoalveolar lavage fluid of patients hospitalized in intensive care units. Pure and silver doped nanoparticles of SiO2 and TiO2 were prepared using a novel modified sol-gel method. Ten clinical strains of P. aeruginosa and the reference PAO1 strain were used. The minimal inhibitory concentration (MIC) was determined by the broth microdilution method. The minimal biofilm inhibitory concentration (MBIC) and biofilm formation were assessed by colorimetric assay. Bacterial enumeration was used to assess the viability of bacteria in the biofilm. Silver nanoparticles immobilized on the SiO2 and TiO2 indicated high antibacterial efficacy against P. aeruginosa planktonic and biofilm cultures. TiO2/Ag0 showed a better bactericidal effect than SiO2/Ag0. Our results indicate that the inorganic compounds (SiO2, TiO2) after nanotechnological modification may be successfully used as antibacterial agents against multidrug-resistant P. aeruginosa strains. |
| Databáze: | OpenAIRE |
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