Laser-capture microdissection of plasma cells from subacute sclerosing panencephalitis brain reveals intrathecal disease-relevant antibodies
| Autor: | Pradeep R. Rai, Alanna M. Ritchie, Carlyne D. Cool, Gregory P. Owens, Donald H. Gilden, Kathryne M. Keays, Mark P. Burgoon |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Adolescent Genetic Vectors Immunoblotting Plasma Cells Cytomegalovirus Subacute sclerosing panencephalitis Immunoglobulin G Cell Line law.invention Measles virus Antigen Antigens CD law medicine Humans ADP-ribosyl Cyclase Microdissection Laser capture microdissection Membrane Glycoproteins Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction Lasers Brain Biological Sciences medicine.disease biology.organism_classification ADP-ribosyl Cyclase 1 Recombinant Proteins Antibodies Anti-Idiotypic Immunology biology.protein Recombinant DNA Subacute Sclerosing Panencephalitis Antibody |
| Zdroj: | Proceedings of the National Academy of Sciences. 102:7245-7250 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0502323102 |
| Popis: | Increased IgG and oligoclonal bands are found in cerebrospinal fluid of humans with chronic infectious CNS disease. Studies have shown that these oligoclonal bands are antibodies directed against the agent that causes disease. Laser-capture microdissection was used to isolate individual CD38+ plasma cells from the brain of a patient with subacute sclerosing panencephalitis, and single-cell RT-PCR was used to analyze individual IgG heavy and light chains expressed by each cell. Based on overrepresented IgG sequences, we constructed functional recombinant antibodies (recombinant IgGs) and determined their specificities. Five of eight recombinant IgGs recognized measles virus, the cause of subacute sclerosing panencephalitis. These results demonstrate that overrepresented IgG sequences in postmortem brains can be used to produce functional recombinant antibodies that recognize their target antigens. This strategy can be used to identify disease-relevant antigens in CNS inflammatory diseases of unknown etiology. |
| Databáze: | OpenAIRE |
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