Subtype-specificity of antipeptide antibodies raised against unique sequences of human interferons-α
| Autor: | Alan R. Hibbs, Gabrielle L. McMullen, Thomas J. Mason, Nison Sattayasai, Maree L. Overall, H. Mario Geysen, Anthony W. Linnane, Paul J. Hertzog, Sangkot Marzuki |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Antigenicity
Blotting Western Molecular Sequence Data Immunology Alpha interferon Enzyme-Linked Immunosorbent Assay Peptide Biology Binding Competitive Epitope law.invention Antibody Specificity law Interferon Sequence Homology Nucleic Acid medicine Humans Amino Acid Sequence Molecular Biology chemistry.chemical_classification Interferon-alpha Recombinant Proteins Blot chemistry Biochemistry Recombinant DNA biology.protein Antibody medicine.drug |
| Zdroj: | Molecular Immunology. 28:975-983 |
| ISSN: | 0161-5890 |
| DOI: | 10.1016/0161-5890(91)90183-k |
| Popis: | A strategy for the production of human interferon-alpha (IFN-alpha) subtype-specific antibodies, based on immunizing rabbits with short unique synthetic peptides coupled to protein carriers, has been validated. These peptides correspond to amino acid residues 99-111 of IFN-alpha 1, 50-57 and 103-116 of IFN-alpha 2, and 37-50 of IFN-alpha 4. The antipeptide antibodies [anti-IFN alpha 1(99-111), anti-IFN alpha 2(50-57C), anti-IFN alpha 2(103-116) and anti-IFN alpha 4(C37-50)] were tested by ELISA and Western blotting for their reactivity with immunoaffinity-purified recombinant human IFN-alpha 1, -alpha 2b and -alpha 4a. The anti-IFN alpha 1(99-111) and anti-IFN alpha 2(50-57C) reacted with their corresponding IFN-alpha and did not crossreact with the other IFN subtypes. The anti-IFN alpha 2(103-116) reacted with IFN-alpha 2b and also crossreacted slightly with the other subtypes. The anti-IFN alpha 4(C37-50) reacted well with IFN-alpha 4a, crossreacted with significantly lower affinity with IFN-alpha 1 and did not bind IFN-alpha 2b. Residues 104-107 and 108-111 are the major components of the epitopes recognized by anti-IFN alpha 1(99-111) and anti-IFN alpha 2(103-116), respectively, as determined by ELISA against overlapping octapeptides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|