Role of Saccharomyces cerevisiae Oxidoreductases Bdh1p and Ara1p in the Metabolism of Acetoin and 2,3-Butanediol
| Autor: | Didac Marco, Eva González, Sylvie Dequin, Josep A. Biosca, Eduard Calam, M. Rosario Fernández, Lauro Sumoy, Xavier Parés |
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| Přispěvatelé: | Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona (UAB), Centro de Regulación Genómica (CRG), Universitat Pompeu Fabra [Barcelona] (UPF), Sciences Pour l'Oenologie (SPO), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Physiology
Dehydrogenase Applied Microbiology and Biotechnology Substrate Specificity chemistry.chemical_compound Anaerobiosis Cloning Molecular Butylene Glycols Conserved Sequence chemistry.chemical_classification [SDV.EE]Life Sciences [q-bio]/Ecology environment 0303 health sciences Ecology biology Acetoin Hydrogen-Ion Concentration Aerobiosis Biochemistry Genetic Engineering Oxidoreductases acetoin Sugar Alcohol Dehydrogenases Biotechnology Saccharomyces cerevisiae Proteins Bdh1p Saccharomyces cerevisiae Alcohol oxidoreductase encoded protein 03 medical and health sciences Ara1p 2 3-Butanediol Histidine 030304 developmental biology 030306 microbiology whole-genome expression 2 3-butanediol NAD biology.organism_classification D-arabinose dehydrogenase Alcohol Oxidoreductases Kinetics Enzyme Amino Acid Substitution chemistry Fermentation Mutation NAD+ kinase Sequence Alignment Gene Deletion Food Science |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2010, 76 (3), pp.670-679. ⟨10.1128/AEM.01521-09⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.01521-09⟩ |
| Popis: | NAD-dependent butanediol dehydrogenase (Bdh1p) from Saccharomyces cerevisiae reversibly transforms acetoin to 2,3-butanediol in a stereospecific manner. Deletion of BDH1 resulted in an accumulation of acetoin and a diminution of 2,3-butanediol in two S. cerevisiae strains under two different growth conditions. The concentrations of (2 R ,3 R )-2,3-butanediol are mostly dependent on Bdh1p activity, while those of ( meso )-2,3-butanediol are also influenced by the activity of NADP(H)-dependent oxidoreductases. One of them has been purified and shown to be d -arabinose dehydrogenase (Ara1p), which converts ( R / S )-acetoin to meso -2,3-butanediol and (2 S ,3 S )-2,3-butanediol. Deletion of BDH2 , a gene adjacent to BDH1 , whose encoded protein is 51% identical to Bdh1p, does not significantly alter the levels of acetoin or 2,3-butanediol in comparison to the wild-type strain. Furthermore, we have expressed Bdh2p with a histidine tag and have shown it to be inactive toward 2,3-butanediol. A whole-genome expression analysis with microarrays demonstrates that BDH1 and BDH2 are reciprocally regulated. |
| Databáze: | OpenAIRE |
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