Microvirga sp. symbiovar mediterranense nodulates Lupinus cosentinii grown wild in Morocco
| Autor: | Hanane Lamin, Hanaa Abdelmoumen, Eulogio J. Bedmar, Salma ElFaik, Alvaro Peix, Mustapha Missbah El Idrissi, Germán Tortosa |
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| Přispěvatelé: | Académie Hassan II des Sciences et Techniques, Junta de Andalucía, European Commission |
| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial Root nodule food.ingredient Microvirga Biology Applied Microbiology and Biotechnology Soil food Phylogenetics RNA Ribosomal 16S Botany Symbiosis Phylogeny Diversity Genes Essential Phylogenetic tree Agriculture Sequence Analysis DNA General Medicine 16S ribosomal RNA rpoB Lupinus Housekeeping gene Morocco Phylogenetic diversity Microbial phylogenetics bacteria Root Nodules Plant Methylobacteriaceae Biotechnology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Aim: To analyse the diversity of nodule-forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results: Of the 31 bacterial strains, four were selected based on their REP-PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii-isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions: To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule-forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study: In this work, we show that Microvirga sp. can be isolated from root nodules of wild-grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected. This project was supported by the Hassan II Academy of Sciences and Techniques (Morocco). Support was also obtained from the Moroccan Ministry of National Education, Vocational Training, Higher Education and Scientific Research within the frame of the ‘Priority projects for scientific research and technological development, PPR2’. Financial support was also obtained from the ERDF-cofinanced project PEAGR2012-1968 granted by Consejería de Economía, Innovacion y Ciencia (Junta de Andalucía, Spain). |
| Databáze: | OpenAIRE |
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