Application of Molecular Beam Deflection Time-of-Flight Mass Spectrometry to Peptide Analysis
Autor: | Philippe Dugourd, Frederick C. Hagemeister, Michel Broyer, David Pippen, Driss Rayane, Michel Rossignol, Martin F. Jarrold, Rodolphe Antoine, I. Compagnon, Nicolas Sommerer |
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Přispěvatelé: | Laboratoire de Spectrométrie Ionique et Moléculaire (LASIM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS) |
Rok vydání: | 2003 |
Předmět: |
Protein Conformation
Molecular Sequence Data Analytical chemistry Peptide Mass spectrometry 01 natural sciences Mass Spectrometry Analytical Chemistry [CHIM.ANAL]Chemical Sciences/Analytical chemistry Electrochemistry Amino Acid Sequence chemistry.chemical_classification Molecular mass 010405 organic chemistry Chemistry Angiotensin II 010401 analytical chemistry Gramicidin Dipeptides Luteinizing Hormone 0104 chemical sciences Molecular Weight Dipole Amino Acids Neutral Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mass spectrum Isobaric process Angiotensin I Time-of-flight mass spectrometry Peptides Molecular beam Algorithms |
Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2003, 75 (20), pp.5512-5516. ⟨10.1021/ac030202o⟩ |
ISSN: | 1520-6882 0003-2700 |
Popis: | International audience; he application of molecular beam deflection time-of-flight mass spectrometry (MBD-TOFMS) to peptide identification is described. The technique permits a simultaneous measurement of molecular mass and electric dipole susceptibility. The mass and susceptibility are not strongly correlated, and the results can be presented as a two-dimensional map. The susceptibility provides a useful way to disperse isobaric and isomeric peptides, and at least for small peptides, the susceptibility is significantly different for different amino acid sequences. Results for peptides in the mass range 1000-2300 Da show that the mass and susceptibility lead to a higher identification score than mass spectra alone. |
Databáze: | OpenAIRE |
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