Seroreactivity toChlamydia trachomatisHsp10 Correlates with Severity of Human Genital Tract Disease
Autor: | David LaVerda, Richard P. Morrison, Lisa N. Albanese, Gerald I. Byrne, Kevin A Ault, Sandra G. Morrison, Paul Ruther |
---|---|
Rok vydání: | 2000 |
Předmět: |
Adult
Infertility Sexually transmitted disease Immunology Population Chlamydia trachomatis Enzyme-Linked Immunosorbent Assay medicine.disease_cause Microbiology Immunopathology Chaperonin 10 medicine Humans Chlamydiaceae education DNA Primers Host Response and Inflammation Antigens Bacterial education.field_of_study Base Sequence biology Chaperonin 60 Chlamydia Infections Tubal factor infertility biology.organism_classification medicine.disease Antibodies Bacterial Infectious Diseases Case-Control Studies Chlamydiales Acute Disease Female Parasitology Infertility Female Bacterial Outer Membrane Proteins |
Zdroj: | Infection and Immunity. 68:303-309 |
ISSN: | 1098-5522 0019-9567 |
Popis: | We have identified the chlamydial heat shock protein Hsp10 as a potential correlate to the immunopathogenic process in women with tubal factor infertility (TFI). The human serologic response to chlamydial Hsp10, Hsp60, and major outer membrane protein (MOMP) was measured by enzyme-linked immunosorbent assay. Three populations of women were studied: uninfected controls (CU), acutely infected (AI) women, and women with TFI. Sera from women in the AI and TFI groups both recognized Hsp10 more frequently and at a higher overall level than sera from healthy uninfected controls. Moreover, the infertile women had significantly greater Hsp10 seroreactivity than acutely infected women, indicating a concomitant increase of Hsp10 recognition in populations with increasing levels of disease severity. Hsp60 reactivity showed a similar correlation in these populations, while MOMP reactivity peaked at the same level in both AI and TFI populations but did not increase with disease severity. Test populations were standardized by level of reactivity to formalin-fixedChlamydia trachomatiselementary bodies (EBs) to address whether these associations were reflections of increased overall chlamydial exposure rather than a property specific to Hsp10. Associations between Hsp10 seropositivity and TFI were greater in the EB+subgroup while associations among the EB−subgroup were diminished. When restricted to the EB+subgroups, Hsp60 and MOMP responses in the TFI population did not increase significantly over the level of AI group responses. Thus, among women with similar exposure to chlamydiae, the serologic response to Hsp10 exhibited a stronger correlation with TFI than did the response to Hsp60 or MOMP. These findings support the hypothesis that the serological response toC. trachomatisheat shock proteins is associated with the severity of disease and identifies Hsp10 as an antigen recognized by a significant proportion of women with TFI. |
Databáze: | OpenAIRE |
Externí odkaz: |