Efficient enantioselective separation of drug enantiomers by immobilised antibody fragments
| Autor: | Hans Söderlund, Kaija Kukkonen, Tuija Teerinen, Lotta Söderholm, Tuula T. Teeri, Tarja K. Nevanen, Markus Linder, Tapani Suortti |
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| Rok vydání: | 2001 |
| Předmět: |
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Chromatography Molecular mass Elution Chemistry Organic Chemistry Enantioselective synthesis Stereoisomerism General Medicine Biochemistry Chromatography Affinity Analytical Chemistry Separation process law.invention Pharmaceutical Preparations Affinity chromatography law Recombinant DNA Enantiomer Immunoglobulin Fragments Chromatography High Pressure Liquid |
| Zdroj: | Nevanen, T, Söderholm, L, Kukkonen, K, Suortti, T, Teerinen, T, Linder, M, Söderlund, H & Teeri, T 2001, ' Efficient enantioselective separation of drug enantiomers by immobilized antibody fragments ', Journal of Chromatography A, vol. 925, no. 1-2, pp. 89-97 . https://doi.org/10.1016/S0021-9673(01)01021-4 |
| ISSN: | 0021-9673 |
| Popis: | There is an increasing need for methods for efficient enantioselective separation and purification of chiral drugs. Genetic engineering provides the means for generating recombinant antibodies exhibiting extremely high specificity for even small molecular mass compounds. Here, recombinant antibody fragments have been generated for the drug diarylalkyltriazole that contains two chiral centres. Immobilised antibody fragments has been used successfully for efficient, step-wise separation of two enantiomers of the drug. Owing to the antibody specificity, one enantiomer came out in the flow-through, while the bound enantiomer could be specifically eluted. One of the antibodies tolerated solvents required both for dissolving the target molecules and for their elution for extended times and was shown to function over multiple cycles of the separation process. |
| Databáze: | OpenAIRE |
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