Detection of Circulating Tissue Factor and Factor VII in a Normal Population
| Autor: | Gabriele Siegert, Thomas Luther, Matthias Kotzsch, Sybille Albrecht, Marianne Grosser, H Grossmann, Martin Müller |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent medicine.drug_class Microgram Population Enzyme-Linked Immunosorbent Assay Monoclonal antibody Thromboplastin Tissue factor chemistry.chemical_compound Sex Factors Internal medicine Blood plasma medicine Animals Humans education Blood Coagulation education.field_of_study Factor VII Chemistry Age Factors Normal population Hematology Middle Aged Endocrinology Female Rabbits |
| Zdroj: | Thrombosis and Haemostasis. 75:772-777 |
| ISSN: | 2567-689X 0340-6245 |
| Popis: | SummaryThe plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation. |
| Databáze: | OpenAIRE |
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