Distribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil

Autor: Marcos Antônio Soares, E C Miranda, Gilvan Ferreira da Silva, R. E. Hanada, Luadir Gasparotto, Casley Borges de Queiroz, Nelcimar Reis Sousa
Rok vydání: 2013
Předmět:
Black sigatoka
Genetic Distance
Minisatellite Repeat
Fungal Protein
Minisatellite Repeats
Polymerase Chain Reaction
Banana
Mat1 1 Gene
Polymorphism (computer science)
Genetic Marker
Allele
Genetics
biology
Inter Simple Sequence Repeat
Bacteriophage Dna
Species Distribution
General Medicine
Fungus Isolation
Genetic Variability
Mycosphaerella
Variable Number Of Tandem Repeat
Brazil
Genetic Markers
Gene Sequence
Microsatellite Dna
Fungal Gene
Plant Disease
Microbiology
Mycosphaerella Fijiensis
Fungal Proteins
Genetic Similarity
Ascomycota
Controlled Study
Plant Leaf
Genetic variability
Molecular Biology
Alleles
Plant Diseases
Polymorphism
Genetic
Mating Type
Dna Fingerprinting
Brasil
Retroposon
Mat1 2 Gene
Methodology
Musa
Nonhuman
Genes
Mating Type
Fungal
biology.organism_classification
Ascomycetes
Fungal Plant Disease
Plant Leaves
Minisatellite
Genetic marker
Geographic Origin
Bacteriophage M13
Microsatellite Repeats
Zdroj: Repositório Institucional do INPA
Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
ISSN: 1676-5680
DOI: 10.4238/2013.february.8.9
Popis: The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates. The fungus Mycosphaerella fijiensis is the causative agent of black sigatoka, which is one of the most destructive diseases of banana plants. Infection with this pathogen results in underdeveloped fruit, with no commercial value. We analyzed the distribution of the M. fijiensis mating-type system and its genetic variability using M13 phage DNA markers. We found a 1:1 distribution of mating-type alleles, indicating MAT1-1 and MAT1-2 idiomorphs. A polymorphism analysis using three different primers for M13 markers showed that only the M13 minisatellite primers generated polymorphic products. We then utilized this polymorphism to characterize 40 isolates from various Brazilian states. The largest genetic distances were found between isolates from the same location and between isolates from different parts of the country. Therefore, there was no correlation between the genetic similarity and the geographic origin of the isolates. The M13 marker was used to generate genetic fingerprints for five isolates; these fingerprints were compared with the band profiles obtained from inter-simple sequence repeat (UBC861) and inter-retrotransposon amplified polymorphism analyses. We found that the M13 marker was more effective than the other two markers for differentiating these isolates.
Databáze: OpenAIRE
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