The sunflower transcription factor HaHB11 confers tolerance to water deficit and salinity to transgenic Arabidopsis and alfalfa plants

Autor: Julieta Virginia Cabello, María Cristina Gomez, Jorge Ignacio Giacomelli, Raquel Lia Chan
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Adaptation
Biological
Arabidopsis
Genetically modified crops
01 natural sciences
Applied Microbiology and Biotechnology
purl.org/becyt/ford/1 [https]
DROUGHT TOLERANCE
Gene Expression Regulation
Plant
VASCULAR BUNDLES
TRANSCRIPTION FACTOR
Biomass
Promoter Regions
Genetic
Plant Proteins
biology
food and beverages
General Medicine
Salt Tolerance
Plants
Genetically Modified
Sunflower
Adaptation
Physiological
Droughts
HAHB11
HOMEODOMAIN-LEUCINE ZIPPER I
Helianthus
CIENCIAS NATURALES Y EXACTAS
Biotechnology
Medicago sativa
Otras Ciencias Biológicas
Transgene
Drought tolerance
Bioengineering
Ciencias Biológicas
03 medical and health sciences
Stress
Physiological
Botany
purl.org/becyt/ford/1.6 [https]
Homeodomain Proteins
Leucine Zippers
Abiotic stress
Arabidopsis Proteins
fungi
Water
biology.organism_classification
Vascular bundle
Salinity
Plant Leaves
030104 developmental biology
Seedlings
SEED YIELD
010606 plant biology & botany
Transcription Factors
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1873-4863
Popis: Homeodomain-leucine zipper (HD-Zip) transcription factors are unique to the plant kingdom; members of subfamily I are known to be involved in abiotic stress responses. HaHB11 belongs to this subfamily and it was previously shown that it is able to confer improved yield and tolerance to flooding via a quiescent strategy. Here we show that HaHB11 expression is induced by ABA, NaCl and water deficit in sunflower seedlings and leaves. Arabidopsis transgenic plants expressing HaHB11, controlled either by its own promoter or by the constitutive 35S CaMV, presented rolled leaves and longer roots than WT when grown under standard conditions. In addition, these plants showed wider stems and more vascular bundles. To deal with drought, HaHB11 transgenic plants closed their stomata faster and lost less water than controls, triggering an enhanced tolerance to such stress condition and also to salinity stress. Concomitantly, ABA-synthesis and sensing related genes were differentially regulated in HaHB11 transgenic plants. Either under long-term salinity stress or mild drought stress, HaHB11 transgenic plants did not exhibit yield penalties. Moreover, alfalfa transgenic plants were generated which also showed enhanced drought tolerance. Altogether, the results indicated that HaHB11 was able to confer drought and salinity tolerance via a complex mechanism which involves morphological, physiological and molecular changes. Fil: Cabello, Julieta Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Giacomelli, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Gómez, María C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Databáze: OpenAIRE
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