Low level LED photodynamic therapy using curcumin loaded tetraether liposomes
| Autor: | Lili Duse, Shashank Reddy Pinnapireddy, Boris Strehlow, Jarmila Jedelská, Udo Bakowsky |
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| Rok vydání: | 2018 |
| Předmět: |
Curcumin
medicine.medical_treatment Perforation (oil well) Pharmaceutical Science Photodynamic therapy Chick Embryo 02 engineering and technology Chorioallantoic Membrane 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Line Tumor medicine Animals Humans Cells Cultured Drug Carriers Liposome Photosensitizing Agents Chemistry Vesicle General Medicine 021001 nanoscience & nanotechnology Chorioallantoic membrane Photochemotherapy Biochemistry 030220 oncology & carcinogenesis Liposomes Biophysics Reactive Oxygen Species 0210 nano-technology Phototoxicity Biotechnology |
| Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 126:233-241 |
| ISSN: | 0939-6411 |
| DOI: | 10.1016/j.ejpb.2017.10.005 |
| Popis: | Oncological use of photodynamic therapy is an evolving field in cancer therapeutics. Photosensitisers are prone to accumulation inside healthy tissues causing undesirable effects. To avoid this, we have developed tetraether lipid liposomal formulations containing curcumin which is a naturally occurring anti-cancer substance and deemed to be safe towards healthy cells. Upon excitation with light at a specific wavelength, curcumin produces reactive oxygen species (ROS) in presence of oxygen, thereby exhibiting a cytotoxic effect towards the surrounding tissues, giving a total control on the onset of therapy. In our study, we examined two different liposomal formulations wherein curcumin is encapsulated within the hydrophobic milieu with the intent to increase its bioavailability. Hydrodynamic diameter, surface charge, stability, morphology and haemocompatibility of the liposomes were studied. The results confirmed the formation of stable nanometre range liposomal vesicles (200-220 nm) containing curcumin which were haemocompatible with coagulation time less than 50 s and a haemolytic potential below 40%. Increased ROS generation post irradiation (>50% compared to un-irradiated samples) was confirmed using fluorescence spectroscopy. The efficiency and selectivity of the PDT was demonstrated by assessing their viability post irradiation and by qualitative analysis using confocal microscopy showing nuclear perforation induced by PDT. Photo-destructive effects of PDT on the microvasculature were studied in vivo using chick chorioallantoic membrane model (CAM). Considerable phototoxicity could be observed in the irradiated area of the CAM 30 min post irradiation. Phototoxic effects in vitro (in SK-OV-3 and PCS-100-020™) and in vivo (in chorioallantoic membrane model) in combination with a novel custom manufactured LED irradiating device showed a formulation dependant selective photodynamic effect of the curcumin liposomes. |
| Databáze: | OpenAIRE |
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