Evaluation of surface plasmon resonance relative to high pressure liquid chromatography for the determination of paralytic shellfish toxins
| Autor: | Barbara Niedzwiadek, Katrina Campbell, Dorothea F. K. Rawn, Christopher T. Elliott, H. Cowan Higgins |
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| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Saxitoxin Chromatography biology Neosaxitoxin General Chemistry Surface Plasmon Resonance medicine.disease High-performance liquid chromatography Shellfish poisoning chemistry.chemical_compound chemistry Seafood Polyclonal antibodies Limit of Detection medicine biology.protein Animals Paralytic shellfish poisoning Surface plasmon resonance General Agricultural and Biological Sciences Chromatography High Pressure Liquid Shellfish |
| Zdroj: | Journal of agricultural and food chemistry. 57(21) |
| ISSN: | 1520-5118 |
| Popis: | A surface plasmon resonance (SPR) method, incorporating monoclonal and polyclonal antibodies, was compared to HPLC fluorescence for the determination of paralytic shellfish toxins (PSTs) in shellfish collected from different regions of Canada (n = 33) and Europe (n = 55). Cross-reactivity between saxitoxin (STX) and its structural analogues was determined for both monoclonal (GT-13A) and polyclonal (R895) antibodies. Method detection limits based on IC(10) values, using the SPR methodology (0.55-71.3 ng/mL), in particular for GT-13A, were somewhat higher than those determined using HPLC (0.16-1.29 ng/mL). SPR analyses generally resulted in higher PST levels relative to those obtained using HPLC, although neither antibody successfully responded to the N-1-hydroxylated analogues (e.g., neosaxitoxin). Five and 10 (R895 and GT-13A, respectively) of the 88 samples tested resulted in PST concentrations above the regulatory limit (80 microg/100 g shellfish tissue as STX equivalents), although HPLC responses indicated that these samples were within acceptable levels. Two and five samples were found to have PST concentrations below the regulatory limit using the GT-13A and R895, respectively, when HPLC results exceeded the limit. SPR may be applicable as a screening technique, although improved antibody response to the N-1-hydroxylated PSTs is required prior to this method being safely used for routine testing. |
| Databáze: | OpenAIRE |
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