Frequency of DLK1 c.639C>T polymorphism and the analysis of MEG3/DLK1/PEG11 cluster expression in muscle of swine raised in Poland

Autor:	M. Różycki, Katarzyna Piórkowska, Barbara Rejduch, Maria Oczkowicz, Katarzyna Ropka-Molik
Rok vydání:	2011
Předmět:	Genetics MEG3 Polymorphism Genetic Swine Muscles Homozygote Gene Expression Muscle Proteins Locus (genetics) Biology Breed Polar overdominance Phenotype Gene Frequency Multigene Family Mutation Gene expression Genotype Animals Gene Silencing Poland Allele Genotyping Alleles Food Science
Zdroj:	Meat Science. 88:627-630
ISSN:	0309-1740
Popis:	DLK1 — (Drosophila like element 1) is a paternally expressed gene, associated with the callipyge phenotype in sheep. In a present study we designed a new real-time PCR alleleic discrimination assay for genotyping of a silent C/T mutation (c.639C>T) in DLK1 gene in swine. The DLK1 c.639C>T mutation was highly polymorphic in all breeds analyzed and C allele was predominant in Landrace and Duroc while T allele was more frequent in Pietrain and Pulawska breed. Moreover, we analyzed mRNA expression of DLK1 and adjacent genes — MEG3 and PEG11 in muscles of swines of different breeds raised in Poland. We did not observe significantly different expression of DLK1 , MEG3 or PEG11 mRNA in any of analyzed breeds. We also attempted to assess the effect of DLK1 (c.639C>T) on the expression of genes in callipyge locus but did not find significant differences between animals with alternate genotypes (C/C and T/T homozygotes).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d722b40f27e87e3415aabb25fa924811 https://doi.org/10.1016/j.meatsci.2011.02.019 Zobrazit plný text záznamu Full Text from ScienceDirect