Quantitation with QUEST of brain HRMAS-NMR signals: Application to metabolic disorders in experimental epileptic seizures

Autor: Danielle Graveron-Demilly, G. Testylier, A. Foquin, P. Carpentier, H. Rabeson, F. Dorandeu, Florence Fauvelle, D. van Ormondt
Přispěvatelé: Centre de Recherche et d'Application en Traitement de l'Image et du Signal (CREATIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Creatis, Compte Général
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Magnetic Resonance Spectroscopy
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
Glutamine
Metabolite
Soman
[INFO.INFO-IM] Computer Science [cs]/Medical Imaging
Acetates
Choline
Mice
chemistry.chemical_compound
Status Epilepticus
Nuclear magnetic resonance
[INFO.INFO-TS]Computer Science [cs]/Signal and Image Processing
Piriform cortex
Magic angle spinning
gamma-Aminobutyric Acid
Alanine
Chemistry
Brain
[PHYS.MECA.ACOU]Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
[INFO.INFO-TI] Computer Science [cs]/Image Processing [eess.IV]
[INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV]
Lactates
[PHYS.PHYS.PHYS-MED-PH]Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph]
[SDV.IB]Life Sciences [q-bio]/Bioengineering
medicine.symptom
[PHYS.MECA.ACOU] Physics [physics]/Mechanics [physics]/Acoustics [physics.class-ph]
Monte Carlo Method
[SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing
Algorithms
[INFO.INFO-TS] Computer Science [cs]/Signal and Image Processing
Status epilepticus
[SDV.IB.MN]Life Sciences [q-bio]/Bioengineering/Nuclear medicine
[SDV.IB.MN] Life Sciences [q-bio]/Bioengineering/Nuclear medicine
medicine
[INFO.INFO-IM]Computer Science [cs]/Medical Imaging
Animals
Radiology
Nuclear Medicine and imaging
[SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing
[SDV.IB] Life Sciences [q-bio]/Bioengineering
[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph]
Aspartic Acid
[SPI.ACOU] Engineering Sciences [physics]/Acoustics [physics.class-ph]
[PHYS.PHYS.PHYS-MED-PH] Physics [physics]/Physics [physics]/Medical Physics [physics.med-ph]
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging
Inositol
Ex vivo
Zdroj: Magnetic Resonance in Medicine
Magnetic Resonance in Medicine, Wiley, 2008, 59 (6), pp.1266--1273
ISSN: 0740-3194
1522-2594
Popis: Quantitation of High Resolution Magic Angle Spinning (HRMAS) Nuclear Magnetic Resonance (NMR) signals enables establishing reference metabolite profiles of ex vivo tissues. Signals are often contaminated by a background signal originating mainly from macromolecules and lipids and by residual water which hampers proper quantitation. We show that automatic quantitation of HRMAS signals, even in the presence of a background, can be achieved by the semi-parametric algorithm QUEST based on prior knowledge of a metabolite basis-set. The latter was quantum-mechanically simulated with NMR-SCOPE and requires accurate spin parameters. The region of interest of spectra is a small part of the full spectral bandwidth. Reducing the computation time inherent to the large number of data-points is possible by using ER-Filter in a preprocessing step. Through Monte-Carlo studies, we analyze the performances of quantitation without and with ER-Filtering. Applications of QUEST to quantitation of 1H ex vivo HRMAS-NMR data of mouse brains after intoxication with soman, are demonstrated. Metabolic profiles obtained during status epilepticus and later when neuronal lesions are installed, are established. Acetate, Alanine, Choline and γ-amino-butyric acid concentrations increase in the piriform cortex during the initial status epilepticus, when seizures are maximum; Lactate and Glutamine concentrations increase while myo-Inositol and N-acetylaspartate concentrations decrease when neuronal lesions are clearly installed. Magn Reson Med 59:1266–1273, 2008. © 2008 Wiley-Liss, Inc.
Databáze: OpenAIRE
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