Effects of Volatile Fatty Acids on Propionate Metabolism and Gluconeogenesis in Caprine Hepatocytes
| Autor: | L.E. Armentano, R.J. Aiello |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Butyrate In Vitro Techniques Valerate chemistry.chemical_compound Isobutyrates Biosynthesis Carnitine Valerates Genetics Animals chemistry.chemical_classification Isovalerate Goats Gluconeogenesis Metabolism Fatty Acids Volatile Amino acid Butyrates Liver chemistry Biochemistry Propionate Animal Science and Zoology Propionates Food Science |
| Zdroj: | Journal of Dairy Science. 70:2504-2510 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.s0022-0302(87)80318-1 |
| Popis: | Isolated caprine hepatocytes were incubated with fatty acids of various chain lengths. Short-chain fatty acids effects on rates of gluconeogenesis and oxidation from [2- 14 C]propionate were determined. Additions of glucose (2.5m M ) had no effect on hepatic [2- 14 C]propionate metabolism in the presence and absence of amino acids. A complete mixture of amino acids increased label incorporation from [2- 14 C]propionate into [ 14 C]glucose by 22%. Butyrate inhibited [2- 14 C]propionate metabolism and increased the apparent Michaelis constant for [2- 14 C]propionate incorporation into [ 14 C]glucose from 2.4±1.5 to 5.6±.9m M . Butyrate's effects on propionate were similar in the presence and absence of L-carnitine (1m M ). Isobutyrate, 2-methylbutyrate, and valerate (1.25m M ) had no effect on [ 14 C]glucose production but decreased 14 CO 2 production to 57, 61, and 54% of the control [2- 14 C]propionate (1.25m M ). This inhibition on 14 CO 2 production was not competitive. Isovalerate had no effect on either [2- 14 C] propionate incorporation into glucose or CO 2 . An increase in ratio of [ 14 C]glucose to 14 CO 2 from [2- 14 C]propionate demonstrated that short-chain fatty acids other than butyrate do not inhibit gluconeogenesis from propionate. In addition, fatty acids that generate a net synthesis of intracellular oxaloacetate may partition propionate carbons toward gluconeogenic rather than oxidative pathways in goat hepatocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|