Inter-laboratory comparison on the size and stability of monodisperse and bimodal synthetic reference particles for standardization of extracellular vesicle measurements
| Autor: | Michael Krumrey, J. Pétry, Felix Meli, Egbert Buhr, Petr Cizmar, Yuana Yuana, Anaïs Nicolet, Bert de Boeck, Edwin van der Pol, Noham Sebaihi, Christian Gollwitzer, Rob H. Bergmans, Rienk Nieuwland, Vincent Fokkema |
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| Přispěvatelé: | Other Research, Biomedical Engineering and Physics, Laboratory for Experimental Clinical Chemistry, Amsterdam Cardiovascular Sciences, Cancer Center Amsterdam |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Applied Mathematics Dispersity Nanoparticle Nanotechnology 02 engineering and technology Extracellular vesicle 021001 nanoscience & nanotechnology 01 natural sciences Stability (probability) Characterization (materials science) Metrology 010309 optics 0103 physical sciences Particle Inter-laboratory 0210 nano-technology Instrumentation Engineering (miscellaneous) Biomedical engineering |
| Zdroj: | MEASUREMENT SCIENCE & TECHNOLOGY, 27(3). IOP Publishing Ltd. |
| DOI: | 10.1088/0957-0233/27/3/035701 |
| Popis: | In future, measurements of extracellular vesicles in body fluids could become a standard diagnostic tool in medicine. For this purpose, reliable and traceable methods, which can be easily applied in hospitals, have to be established. Within the European Metrological Research Project (EMRP) 'Metrological characterization of micro-vesicles from body fluids as non-invasive diagnostic biomarkers' (www.metves.eu), various nanoparticle reference materials were developed and characterized. We present results of an international comparison among four national metrology institutes and a university hospital. The size distributions of five monodisperse and two bimodal spherical particle samples with diameters ranging from 50 nm to 315 nm made out of silica and polystyrene were compared. Furthermore, the stability of the samples was verified over a period of 18 months. While monodisperse reference particle samples above a certain size level lead to good agreements of the size measurements among the different methods, small and bimodal samples show the limitations of current 'clinical' methods. All samples proved to be stable within the uncertainty of the applied methods. |
| Databáze: | OpenAIRE |
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