Vesicular trafficking plays a role in centriole disengagement and duplication
| Autor: | Benjamin J. Nichols, Gregory C. Rogers, Steven H Caplan, Kriti Bahl, Trey Farmer, Shuwei Xie, Ivana Yeow, Naava Naslavsky, Tiffany A. McLamarrah, James B. Reinecke |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Centriole Endocytic cycle Vesicular Transport Proteins Cell Cycle Proteins Nerve Tissue Proteins Centrosome cycle Biology 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Ciliogenesis Humans Antigens Microtubule anchoring Molecular Biology Centrioles Cytokinesis Brief Report Cytoplasmic Vesicles Intracellular Signaling Peptides and Proteins Transferrin Cell Biology Endocytosis Cell biology Protein Transport Midbody 030104 developmental biology Mitotic exit Centrosome 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e18-04-0241 |
| Popis: | Centrosomes are the major microtubule-nucleating and microtubule-organizing centers of cells and play crucial roles in microtubule anchoring, organelle positioning, and ciliogenesis. At the centrosome core lies a tightly associated or “engaged” mother–daughter centriole pair. During mitotic exit, removal of centrosomal proteins pericentrin and Cep215 promotes “disengagement” by the dissolution of intercentriolar linkers, ensuring a single centriole duplication event per cell cycle. Herein, we explore a new mechanism involving vesicular trafficking for the removal of centrosomal Cep215. Using small interfering RNA and CRISPR/Cas9 gene-edited cells, we show that the endocytic protein EHD1 regulates Cep215 transport from centrosomes to the spindle midbody, thus facilitating disengagement and duplication. We demonstrate that EHD1 and Cep215 interact and show that Cep215 displays increased localization to vesicles containing EHD1 during mitosis. Moreover, Cep215-containing vesicles are positive for internalized transferrin, demonstrating their endocytic origin. Thus, we describe a novel relationship between endocytic trafficking and the centrosome cycle, whereby vesicles of endocytic origin are used to remove key regulatory proteins from centrosomes to control centriole duplication. |
| Databáze: | OpenAIRE |
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