Microbial diversity and community structure of a highly active anaerobic methane-oxidizing sulfate-reducing enrichment
| Autor: | Stefan Schouten, G. Christian Jagersma, Piet N.L. Lens, Jarno Gieteling, Alfons J. M. Stams, Adam Klimiuk, Roel J.W. Meulepas, Ineke Heikamp-de Jong, Jaap S. Sinninghe Damsté |
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| Rok vydání: | 2009 |
| Předmět: |
Geologic Sediments
archaea oxidation Flavobacteriales sequence data in-vitro Microbiology chemistry.chemical_compound Bioreactors black-sea mud volcano Microbiologie RNA Ribosomal 16S Bioreactor Seawater Anaerobiosis Sulfate Sulfate-reducing bacteria bacteria Ecology Evolution Behavior and Systematics Archaeol Phylogeny cold-seep WIMEK biology Sulfur-Reducing Bacteria 16s ribosomal-rna Sulfates Biodiversity biology.organism_classification Archaea marine-sediments Biochemistry chemistry Environmental chemistry Anaerobic oxidation of methane Environmental Technology Milieutechnologie Methane Bacteria |
| Zdroj: | Environmental Microbiology, 11(12), 3223-3232 Environmental Microbiology 11 (2009) 12 |
| ISSN: | 1462-2920 1462-2912 |
| Popis: | Summary Anaerobic oxidation of methane (AOM) is an important methane sink in the ocean but the microbes responsible for AOM are as yet resilient to cultivation. Here we describe the microbial analysis of an enrichment obtained in a novel submerged-membrane bioreactor system and capable of high-rate AOM (286 mumol g(dry weight) (-1) day(-1)) coupled to sulfate reduction. By constructing a clone library with subsequent sequencing and fluorescent in situ hybridization, we showed that the responsible methanotrophs belong to the ANME-2a subgroup of anaerobic methanotrophic archaea, and that sulfate reduction is most likely performed by sulfate-reducing bacteria commonly found in association with other ANME-related archaea in marine sediments. Another relevant portion of the bacterial sequences can be clustered within the order of Flavobacteriales but their role remains to be elucidated. Fluorescent in situ hybridization analyses showed that the ANME-2a cells occur as single cells without close contact to the bacterial syntrophic partner. Incubation with (13)C-labelled methane showed substantial incorporation of (13)C label in the bacterial C(16) fatty acids (bacterial; 20%, 44% and 49%) and in archaeal lipids, archaeol and hydroxyl-archaeol (21% and 20% respectively). The obtained data confirm that both archaea and bacteria are responsible for the anaerobic methane oxidation in a bioreactor enrichment inoculated with Eckernförde bay sediment |
| Databáze: | OpenAIRE |
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