β-Catenin/Tcf signaling in murine oocytes identifies nonovulatory follicles
| Autor: | Aida Rizk, Riaz Farookhi, Macalister Usongo |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Ovulation Genetically modified mouse Embryology Estrous Cycle Mice Transgenic Ovary Biology Mice Endocrinology Ovarian Follicle Follicular phase medicine Animals Wnt Signaling Pathway beta Catenin Estrous cycle Wnt signaling pathway Obstetrics and Gynecology Cell Biology beta-Galactosidase Oocyte Antral follicle Cell biology medicine.anatomical_structure Reproductive Medicine Oocytes Female Signal transduction TCF Transcription Factors Biomarkers Gonadotropins |
| Zdroj: | REPRODUCTION. 144:669-676 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-12-0291 |
| Popis: | WNTS are secreted glycoprotein molecules that signal through one of three signaling pathways. The best-characterized pathway involves stabilization of the multifunctional protein β-catenin, which in concert with members of the T-cell factor (Tcf) family activates specific gene transcription. We have examined putative Wnt/β-catenin in the murine ovary using transgenic mice harboring a reporter construct that activates β-galactosidase (lacZ) expression in response to β-catenin/Tcf binding (TopGal mice). Primordial and primary follicles did not stain for lacZ, and the proportion of β-catenin/Tcf signaling oocytes was lower than that of nonsignaling oocytes throughout estrous cycle. β-Catenin/Tcf signaling oocytes were observed in follicles from the secondary stage of development and their proportion increased with follicular maturation (secondary follicles, 20%; early antral and antral follicles, 70%). In contrast, the majority (>90%) of ovulated oocytes did not stain for lacZ. As the oocyte possesses components for WNT signal transduction, our data suggest that β-catenin/Tcf signaling is involved in the development of follicular ovulatory capability and identifies nonovulatory follicles. |
| Databáze: | OpenAIRE |
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