A simple and sensitive bioassay for the detection of human interleukin-18/interferon-gamma-inducing factor using human myelomonocytic KG-1 cells
| Autor: | Mutsuko Taniguchi, Kaori Konishi, Masashi Kurimoto, Kunzo Orita, Fujimi Tanabe, Hiroshi Yamauchi, Masao Ikeda, Tadao Tanimoto |
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| Rok vydání: | 1998 |
| Předmět: |
Lysis
Interferon Inducers medicine.medical_treatment Immunology Biology Cross Reactions Sensitivity and Specificity Interferon-gamma Mice Interferon γ medicine Tumor Cells Cultured Immunology and Allergy Bioassay Animals Humans Secretion Interleukin-18 Molecular biology Cytokine biology.protein Cytokines Interleukin 18 Biological Assay Antibody Myelomonocytic cell |
| Zdroj: | Journal of immunological methods. 209(2) |
| ISSN: | 0022-1759 |
| Popis: | Interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) is a novel cytokine which plays an important role in Th1 responses. Here we describe a simple, sensitive bioassay for human IL-18 using the human myelomonocytic cell line, KG-1, which produces IFN-gamma in response to human IL-18. IFN-gamma production induced by human IL-18 was completely blocked by an antibody against human IL-18. Human IL-18 could be measured in a concentration range from approximately 100 to 10,000 pg/ml, and intra- and inter-assay coefficient variations were both below 15%. It was possible to measure human IL-18 in human serum, cell lysate or culture supernatant by this bioassay. Thus, the human IL-18 bioassay can be expected to be useful in the investigation of the relationship between human IL-18 and various diseases or in analyzing the mechanisms of human IL-18 secretion from IL-18 producing cells. |
| Databáze: | OpenAIRE |
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