Impaired extinction of cued fear memory and abnormal dendritic morphology in the prelimbic and infralimbic cortices in VPAC2 receptor (VIPR2)-deficient mice
| Autor: | Ryosuke Yamauchi, Shuto Takeuchi, Michael S. Fanselow, Abha K. Rajbhandari, Takuya Kawanai, Jesse D. Cushman, James A. Waschek, Atsuko Hayata-Takano, Hitoshi Hashimoto, Yukio Ago |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Conditioning Classical Vasoactive intestinal peptide Dendritic morphology Inbred C57BL Medical and Health Sciences Extinction Psychological Sholl analysis Mice Behavioral Neuroscience 0302 clinical medicine Receptors Fear conditioning Mice Knockout Fear Extinction Mental Health medicine.anatomical_structure Neurological Receptors Vasoactive Intestinal Peptide Type II Cues Psychology Vasoactive Intestinal Peptide VIPR2 1.1 Normal biological development and functioning Knockout Cognitive Neuroscience Infralimbic cortex Prefrontal Cortex Neuropeptide Experimental and Cognitive Psychology Behavioral Science & Comparative Psychology Basic Behavioral and Social Science Type II Article 03 medical and health sciences Underpinning research Behavioral and Social Science medicine Animals Prelimbic and infralimbic cortices Psychology and Cognitive Sciences Neurosciences Dendrites Extinction (psychology) Classical Mice Inbred C57BL 030104 developmental biology Psychological Neuroscience 030217 neurology & neurosurgery Conditioning VPAC2 receptor Basolateral amygdala |
| Zdroj: | Neurobiology of Learning and Memory. 145:222-231 |
| ISSN: | 1074-7427 |
| Popis: | The structurally related neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) have been implicated in stress regulation and learning and memory. Several bodies of research have shown the impact of the PACAP specific receptor PAC1 on fear memory, but the roles of other PACAP receptors in regulating fear stress responses remain to be elucidated. Here we aimed to investigate the effects of genetic deletion of VIPR2 encoding the VPAC2 receptor, which binds both VIP and PACAP, on fear-related memory and on dendritic morphology in the brain regions of the fear circuitry. Male VPAC2 receptor knockout (VPAC2-KO) and littermate wild-type control mice were subjected to Pavlovian fear conditioning paradigm. VPAC2-KO mice displayed normal acquisition of fear conditioning, contextual and cued fear memory, but impaired extinction of cued fear memory. Morphological analyses revealed reductions in cell body size and total branch number and length of apical and basal dendrites of prelimbic cortex neurons in VPAC2-KO mice. In addition, Sholl analysis indicated that the amount of dendritic material distal to the soma was decreased, while proximal dendritic material was increased. In the infralimbic cortex, the amount of apical dendritic material proximal to the soma was increased in VPAC2-KO mice, while other indices of axonal morphology did not differ. Finally, there were no differences in dendritic morphology in basolateral amygdala neurons between genotypes. These findings suggest that the VPAC2 receptor plays an important role in the fear extinction processes and the regulation of the dendritic morphology in the prelimbic and infralimbic cortices. |
| Databáze: | OpenAIRE |
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