Reduced Granulation Tissue and Wound Strength in the Absence of α11β1 Integrin
| Autor: | Beate Eckes, Anja Niehoff, Ida Wiig Sørensen, Jan-Niklas Schulz, Sergio Carracedo, Cédric Zeltz, Ralf Hallinger, Donald Gullberg, Malgorzata Barczyk |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Integrins MAP Kinase Kinase 4 Biochemistry Collagen receptor Mice Transforming Growth Factor beta Myofibroblasts Cells Cultured granulation tissue Mice Knockout MEF biology integumentary system Chemistry Granulation tissue Cell Differentiation α-SMA Cell biology medicine.anatomical_structure GT Models Animal α-smooth muscle actin Female Original Article Collagen Signal transduction Myofibroblast transforming growth Signal Transduction TGF-β Receptors Collagen Integrin Dermatology In Vitro Techniques Cicatrix factor-β In vivo Medisinske Fag: 700 [VDP] Tensile Strength medicine Animals Molecular Biology Wound Healing Transforming growth factor beta Cell Biology Mice Inbred C57BL mouse embryonic fibroblast Immunology Granulation Tissue biology.protein Wound healing |
| Zdroj: | The Journal of Investigative Dermatology |
| ISSN: | 0022-202X |
| DOI: | 10.1038/jid.2015.24 |
| Popis: | Previous wound healing studies have failed to define a role for either α1β1 or α2β1 integrin in fibroblast-mediated wound contraction, suggesting the involvement of another collagen receptor in this process. Our previous work demonstrated that the integrin subunit α11 is highly induced during wound healing both at the mRNA and protein level, prompting us to investigate and dissect the role of the integrin α11β1 during this process. Therefore, we used mice with a global ablation of either α2 or α11 or both integrin subunits and investigated the repair of excisional wounds. Analyses of wounds demonstrated that α11β1 deficiency results in reduced granulation tissue formation and impaired wound contraction, independently of the presence of α2β1. Our combined in vivo and in vitro data further demonstrate that dermal fibroblasts lacking α11β1 are unable to efficiently convert to myofibroblasts, resulting in scar tissue with compromised tensile strength. Moreover, we suggest that the reduced stability of the scar is a consequence of poor collagen remodeling in α11-/- wounds associated with defective transforming growth factor-β–dependent JNK signaling. publishedVersion |
| Databáze: | OpenAIRE |
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