Anticonvulsant activity of β-caryophyllene against pentylenetetrazol-induced seizures
| Autor: | Thaíze Lopes de Souza, Cleide Correia de Oliveira, Ana Flávia Furian, Jéssica Grigoletto, Ana Claudia Beck Grauncke, Irwin Rose Alencar de Menezes, Clarissa Vasconcelos de Oliveira, Leandro Rodrigo Ribeiro, Naieli Schiefelbein Souto, Vinícius Rafael Funck, Mauro Schneider Oliveira |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Clinical Neurology Convulsants Epilepsies Myoclonic Motor Activity Pharmacology medicine.disease_cause Neuroprotection Mice 03 medical and health sciences Behavioral Neuroscience Epilepsy 0302 clinical medicine Seizures Convulsion medicine Animals Pentylenetetrazol Adverse effect Postural Balance Swimming Polycyclic Sesquiterpenes Dose-Response Relationship Drug Chemistry Electroencephalography Recognition Psychology medicine.disease Mice Inbred C57BL Oxidative Stress 030104 developmental biology Anticonvulsant Neurology Anesthesia Pentylenetetrazole Anticonvulsants Neurology (clinical) medicine.symptom Sesquiterpenes 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | Epilepsy & Behavior. 56:26-31 |
| ISSN: | 1525-5050 |
| DOI: | 10.1016/j.yebeh.2015.12.040 |
| Popis: | Increasing evidence suggests that plant-derived extracts and their isolated components are useful for treatment of seizures and, hence, constitute a valuable source of new antiepileptic drugs with improved efficacy and better adverse effect profile. β-Caryophyllene is a natural bicyclic sesquiterpene that occurs in a wide range of plant species and displays a number of biological actions, including neuroprotective activity. In the present study, we tested the hypothesis that β-caryophyllene displays anticonvulsant effects. In addition, we investigated the effect of β-caryophyllene on behavioral parameters and on seizure-induced oxidative stress. Adult C57BL/6 mice received increasing doses of β-caryophyllene (0, 10, 30, or 100mg/kg). After 60 min, we measured the latencies to myoclonic and generalized seizures induced by pentylenetetrazole (PTZ, 60 mg/kg). We found that β-caryophyllene increased the latency to myoclonic jerks induced by PTZ. This result was confirmed by electroencephalographic analysis. In a separate set of experiments, we found that mice treated with an anticonvulsant dose of β-caryophyllene (100mg/kg) displayed an improved recognition index in the object recognition test. This effect was not accompanied by behavioral changes in the open-field, rotarod, or forced swim tests. Administration of an anticonvulsant dose of β-caryophyllene (100mg/kg) did not prevent PTZ-induced oxidative stress (i.e., increase in the levels of thiobarbituric acid-reactive substances or the decrease in nonprotein thiols content). Altogether, the present data suggest that β-caryophyllene displays anticonvulsant activity against seizures induced by PTZ in mice. Since no adverse effects were observed in the same dose range of the anticonvulsant effect, β-caryophyllene should be further evaluated in future development of new anticonvulsant drugs. |
| Databáze: | OpenAIRE |
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