Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury

Autor: Jelena Repac, Dragana Vučević, Biljana Draskovic Pavlovic, Sanja Pekovic, Sanja Dacic, Biljana Bozic Nedeljkovic, Adil Ehmedah, Predrag Nedeljkovic
Rok vydání: 2019
Předmět:
Anti-Inflammatory Agents
Pharmaceutical Science
Fluorescent Antibody Technique
Inflammation
vitamin B complex therapy
Pharmacology
Article
Analytical Chemistry
Proinflammatory cytokine
neuroinflammation
03 medical and health sciences
0302 clinical medicine
Neuroinflammation
Peripheral Nerve Injuries
calcium channels
Drug Discovery
Regeneration
Medicine
peripheral nerve injury
Physical and Theoretical Chemistry
Axon
030304 developmental biology
Vitamin B complex therapy
0303 health sciences
business.industry
Macrophages
Organic Chemistry
Macrophage Activation
Neuroregeneration
Calcium channels
B vitamins
medicine.anatomical_structure
Gene Expression Regulation
Chemistry (miscellaneous)
Peripheral nerve injury
regeneration
Vitamin B Complex
Molecular Medicine
Cytokines
M1/M2 macrophages
Calcium
medicine.symptom
business
030217 neurology & neurosurgery
Reinnervation
Zdroj: Molecules
Volume 24
Issue 24
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Popis: Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.
Databáze: OpenAIRE
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