Performance of TaqMan probes for the detection of sexually transmitted infections in South African women
| Autor: | Ravesh Singh, Nigel Garrett, Koleka Mlisana, Veron Ramsuran, Nireshni Mitchev, Abraham J. Niehaus |
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| Rok vydání: | 2019 |
| Předmět: |
Vaginal discharge
TaqMan Clinical Biochemistry vaginal discharge syndrome urologic and male genital diseases medicine.disease_cause molecular diagnostics Multiplex polymerase chain reaction medicine taqman sexually transmitted infections validation biology business.industry Brief Report Public Health Environmental and Occupational Health biology.organism_classification Molecular diagnostics Virology Medical Laboratory Technology Neisseria gonorrhoeae Trichomonas vaginalis Public aspects of medicine RA1-1270 medicine.symptom business Chlamydia trachomatis Mycoplasma genitalium |
| Zdroj: | African Journal of Laboratory Medicine African Journal of Laboratory Medicine, Vol 10, Iss 1, Pp e1-e4 (2021) |
| ISSN: | 2225-2002 |
| Popis: | Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis and Mycoplasma genitalium are the four main aetiologies of sexually transmitted infections responsible for vaginal discharge syndrome (VDS). Commercially available multiplex polymerase chain reaction (PCR) assays are expensive and generally not customisable. We evaluated a highly customisable singleplex PCR approach by testing it in parallel with the Anyplex™ II STI-7 detection assay in a cohort of South African women that presented with VDS between May 2016 and January 2017. Our multiple singleplex PCR strategy proved to be a simple, accurate, rapid, affordable and scalable option for diagnosing VDS. |
| Databáze: | OpenAIRE |
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