Synthesis and secretion of alkaline phosphatase in vitro from first-trimester and term human placentas
| Autor: | H. Galski, S E Fridovich, Avraham Hochberg, D. Weinstein, N. de Groot, S. Segal, R. Folman |
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| Rok vydání: | 1981 |
| Předmět: |
Placenta
Pregnancy Trimester Third Phosphatase Biology Pregnancy Proteins Organ culture Biochemistry Organ Culture Techniques Pregnancy medicine Humans Secretion Molecular Biology Incubation Cell Biology Alkaline Phosphatase Molecular biology In vitro Isoenzymes Pregnancy Trimester First medicine.anatomical_structure Alkaline phosphatase Female Intracellular Subcellular Fractions Research Article |
| Zdroj: | The Biochemical journal. 194(3) |
| ISSN: | 0264-6021 |
| Popis: | The synthesis and secretion of alkaline phosphatases in vitro by human placental tissue incubated in organ culture were studied. First-trimester placenta synthesizes and secretes two different alkaline phosphatase isoenzymes (heat-labile and heat-stable), whereas in term placenta nearly all the alkaline phosphatase synthesized and secreted is heat-stable. The specific activities of alkaline phosphatases in first-trimester and term placental tissue remain constant throughout the time course of incubation. In the media, specific activities increase with time. Hence, alkaline phosphatase synthesis seems to be the driving force for its own secretion. The rates of synthesis de novo and of alkaline phosphatases were measured. The specific radioactivities of the secreted alkaline phosphatases were higher than the corresponding specific radioactivities in the tissue throughout the entire incubation period. The intracellular distribution of the alkaline phosphatase isoenzymes was compared. |
| Databáze: | OpenAIRE |
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