Anti-tumour activity of longikaurin A (LK-A), a novel natural diterpenoid, in nasopharyngeal carcinoma
| Autor: | Ze Dong Hu, Ji Ke Du, Man Zhi Li, Yong Du, Qing Feng Zou, Mu Sheng Zeng, Juan Zou, Jian-Xin Pu, Hua Zhang, Shu Peng Chen, Dan Xie, Han-Dong Sun, Hong Bo Wang |
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| Rok vydání: | 2013 |
| Předmět: |
Longikaurin A (LK-A)
Cell cycle checkpoint Cell Survival Nasopharyngeal neoplasm Mice Nude Apoptosis Antineoplastic Agents Caspase 3 Biology General Biochemistry Genetics and Molecular Biology Amino Acid Chloromethyl Ketones S Phase Flow cytometry Mice In vivo Cell Line Tumor medicine Animals Tumor Stem Cell Assay Medicine(all) Nasopharyngeal Carcinoma medicine.diagnostic_test Biochemistry Genetics and Molecular Biology(all) Cell growth Research Carcinoma Nasopharyngeal Neoplasms General Medicine Caspase Xenograft Model Antitumor Assays Molecular biology Caspase 9 In vitro Up-Regulation Poly(ADP-ribose) Polymerases Apoptosis Regulatory Proteins Diterpenes Kaurane Signal Transduction |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| DOI: | 10.1186/1479-5876-11-200 |
| Popis: | Background Longikaurin A is a natural ent-kaurene diterpenoid isolated from Isodon genus. The ent-kaurene diterpenoids isolated from medicinal plants have been shown to have anti-disease effects. The present study was designed to examine the anti-tumour effects of longikaurin A (LK-A) in nasopharyngeal carcinoma in vitro and in vivo. Methods Apoptosis and cell cycle arrest were determined by flow cytometry analysis of the cells treated with Longikaurin A. The proteins of apoptosis signaling pathway were detected by western blotting analysis. Finally, we examined whether LK-A exhibits anti-tumour activity in xenograft models. Results Longikaurin A inhibited the cell growth by inducing apoptosis and cell cycle arrest. At low concentrations, longikaurin A induced S phase arrest and at higher concentrations, longikaurin A induced caspase-dependent apoptosis by regulating apoptotic molecules. Finally, longikaurin A significantly inhibited the tumour growth of CNE2 xenografts in vivo and showed no obvious effect on the body weights of the mice. Conclusion Our results suggest that Longikaurin A exhibited anti-tumour activity in nasopharyngeal carcinoma in vitro and in vivo. |
| Databáze: | OpenAIRE |
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