Evaluation of the occurrence of sporulating and nonsporulating pathogenic bacteria in manure and in digestate of five agricultural biogas plants

Autor: A.M. Pourcher, Frédéric Barbut, Evelyne Boscher, Martine Denis, Elisabeth Repérant, Christine Ziebal, Catherine Houdayer, Sophie Le Roux, Céline Druilhe, Sandra Rouxel, Caroline Le Maréchal, Bérengère Nagard, Typhaine Poezevara
Přispěvatelé: Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Optimisation des procédés en Agriculture, Agroalimentaire et Environnement (UR OPAALE), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), CHU Saint-Antoine [APHP]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
anaerobic digestion
Veterinary medicine
BIOGAZ
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
DIGESTAT
Colony Count
Microbial
lcsh:QR1-502
BACTERIE
Biology
medicine.disease_cause
7. Clean energy
Microbiology
lcsh:Microbiology
03 medical and health sciences
Enterobacteriaceae
Biogas
Listeria monocytogenes
Salmonella
medicine
biogas
farmyard manure
bacteria
DIGESTION ANAEROBIE
030304 developmental biology
Spores
Bacterial
Clostridium
2. Zero hunger
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
0303 health sciences
030306 microbiology
LISTERIA MONOCYTOGENES
Campylobacter
Original Articles
Clostridium perfringens
Manure
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Anaerobic digestion
13. Climate action
Biofuels
digestate
Digestate
Clostridium botulinum
Original Article
France
biogas plants
FUMIER
Zdroj: MicrobiologyOpen
MicrobiologyOpen, Wiley, 2019, 8 (10), pp.e872. ⟨10.1002/mbo3.872⟩
MicrobiologyOpen, Vol 8, Iss 10, Pp n/a-n/a (2019)
ISSN: 2045-8827
DOI: 10.1002/mbo3.872⟩
Popis: The number of agricultural biogas plants has been increasing in the past decades in some European countries. Digestates obtained after anaerobic digestion (AD) of manure are usually spread on agricultural land; however, their hygiene status regarding pathogens posing public health and/or animal health challenges has been poorly characterized up to now in France. In this study, three replicates of manure and digestate were collected from five farm biogas plants receiving animal manure in order to assess the occurrence and concentrations of sporulating (Clostridium botulinum, Clostridioides difficile, Clostridium perfringens) and nonsporulating (Listeria monocytogenes, thermotolerant Campylobacter spp., Salmonella, Escherichia coli, enterococci) bacteria. Concentrations of E. coli, enterococci, and C. perfringens in digestates ranged from 102 to 104, 104 to 105, and 7 × 102 MPN/g and from 1.3 to 3 × 102 MPN/g, respectively. Thermotolerant Campylobacter, detected in all the manures, was only found in two digestates at a concentration of cells ranging from
The goal of this study was to assess the occurrence and concentrations of sporulating (Clostridium botulinum, Clostridioides difficile, Clostridium perfringens) and nonsporulating (Listeria monocytogenes, thermotolerant Campylobacter spp., Salmonella, Escherichia coli, enterococci) bacteria in manure and digestate collected in agricultural biogas plants. The levels of pathogenic bacteria in both manure and digestate suggested that some bacteria can persist throughout anaerobic digestion.
Databáze: OpenAIRE
Externí odkaz: