Integration of the SMXL/D53 strigolactone signalling repressors in the model of shoot branching regulation in Pisum sativum
| Autor: | Stephanie C. Kerr, Julie Saffar, Elizabeth A. Dun, Catherine Rameau, Jean-Paul Pillot, Suyash Bhimgonda Patil, Yannick Bellec, Christine A. Beveridge, Sylvie Citerne, Yasmine Ligerot, Alexandre de Saint Germain, Grégoire Aubert |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Cytokinins Mutant Arabidopsis Strigolactone Plant Science 01 natural sciences Lactones 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Loss of Function Mutation Auxin Gene expression Genetics Arabidopsis thaliana Gene Phylogeny Plant Proteins 030304 developmental biology Feedback Physiological 2. Zero hunger chemistry.chemical_classification 0303 health sciences Indoleacetic Acids biology Peas food and beverages Oryza Cell Biology Plants Genetically Modified biology.organism_classification Cell biology chemistry Cytokinin Co-Repressor Proteins Heterocyclic Compounds 3-Ring Signal Transduction 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 107:1756-1770 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/tpj.15415 |
| Popis: | DWARF53 (D53) in rice (Oryza sativa) and its homologs in Arabidopsis (Arabidopsis thaliana), SUPPRESSOR OF MAX2-LIKE 6 (SMXL6), SMXL7 and SMXL8, are well established negative regulators of strigolactone signalling involved in shoot branching. Little is known of pea (Pisum sativum) homologs and whether D53 and related SMXLs are specific to strigolactone signalling pathways. Here, we identify two allelic pea mutants, dormant3 (dor3), and demonstrate through gene mapping and sequencing that DOR3 corresponds to a homolog of D53 and SMXL6/SMXL7, designated PsSMXL7. Phenotype analysis, gene expression, protein and hormone quantification assays were performed to determine the role of PsSMXL7 in bud outgrowth regulation and the role of PsSMXL7 and D53 in integrating strigolactone and cytokinin responses. Like D53 and related SMXLs, we show that PsSMXL7 is strigolactone degradable, and induces feedback upregulation of PsSMXL7 transcript. Here we reveal a system conserved in pea and rice, whereby CK also upregulates PsSMXL7/D53 transcripts, providing a clear mechanism for strigolactone and cytokinin cross-talk in the regulation of branching. Further deepening our understanding of the branching network in pea, we provide evidence that strigolactone acts via PsSMXL7 to modulate auxin content via PsAFB5, which itself regulates expression of strigolactone biosynthesis genes. We therefore show that PsSMXL7 is key to a triple hormone network involving an auxin-SL feedback mechanism and SL-CK crosstalk. |
| Databáze: | OpenAIRE |
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