In-vitro cytocompatibility and growth factor content of GBR/GTR membranes
| Autor: | Julia Saliter, Kristian Unger, Thomas Spinell, Brigitte Hackl, Reinhard Hickel, Matthias Folwaczny |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Periodontal ligament stem cells Periodontal Ligament medicine.medical_treatment Cell Culture Techniques 02 engineering and technology Cytocompatibility Biocompatibility Cell Culture Mesenchymal Stem Cells Periodontal Ligament Cells Cytotoxicity Cell Proliferation Guided Bone Regeneration Guided Tissue Regeneration Membranes Extracellular matrix 03 medical and health sciences 0302 clinical medicine medicine Humans General Materials Science General Dentistry Cell growth Growth factor Mesenchymal stem cell Membranes Artificial 030206 dentistry 021001 nanoscience & nanotechnology Molecular biology In vitro Membrane Mechanics of Materials Cell culture 0210 nano-technology |
| Zdroj: | Dent. Mater. 35, 963-969 (2019) |
| Popis: | Objective. To assess the cytocompatibility of five commercially available xenogenic barrier membranes used for oral regenerative procedures and to determine the growth factor content of these membranes in-vitro.Methods. Human mesenchymal stem cells (hMSCs) and immortalized periodontal ligament stem cells (PDL-hTERTs) were used to determine the cytocompatibility of xenogenic barrier membranes made of collagen (Biogide, BG, Geistlich Pharma AG, Switzerland; Biomend, BM, Zimmer Biomet, USA; Osseoguard OG, Zimmer Biomet, USA; OssixPlus, OX, Datum Dental, Israel) or extracellular matrix (ECM) (Dynamatrix, DM, Keystone Dental, USA) and of their eluates obtained by washing. Cells were cultured with previously washed and unwashed membranes (n=4) and in the medium used for washing (eluate). Cell proliferation at 3 days (eluates) and at 7 days (membranes) was assessed using the WST-1 cell proliferation kit. Growth factor content of the membranes was measured using multiplex ELISA.Results. The eluate of BG and BM significantly inhibited proliferation of hMSCs, whereas DM and OX showed stimulating effects. The highest impact was observed for DM, its eluate doubled the cell proliferation of adherent cells when compared to the control (p0.05). The presence of membranes had different impact on hMSCs and PDLs. hMSCs seem to be more resistant to the inhibitory effects of BG, OG and BM. hMSCs are only affected by OX, which actually stimulates hMSCs when the specimens are not washed previously. PDLs however proliferate significantly less once they are placed into culture with BM and OG as well as BG-not washed. Once BG is washed no inhibitory effect on PDLs was observed, however overall the washing of membrane samples prior to the placement into the cell culture did hardly have any effect on the outcome. The strongest inhibition of proliferation was shown with the BM and OG membrane in PDL-hTERTs (p |
| Databáze: | OpenAIRE |
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