Micro RNA-146a But Not IRAK1 is Associated with Rheumatoid Arthritis in the Tunisian Population
| Autor: | Imen Boussaid, Hana Ben Hassine, Asma Boumiza, Elyes Bouajina, Khadija Baccouche, Ramzi Zemni, Rim Sghiri, Zahid Shakoor, Ahlem Atig |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Tunisia Genotype Biology Polymorphism Single Nucleotide Proinflammatory cytokine Arthritis Rheumatoid 03 medical and health sciences Gene Frequency Rheumatoid Factor Gene expression medicine Rheumatoid factor Humans Genetic Predisposition to Disease Receptor Genetics (clinical) Alleles IRAK1 General Medicine Middle Aged medicine.disease MicroRNAs 030104 developmental biology C-Reactive Protein Interleukin-1 Receptor-Associated Kinases Rheumatoid arthritis Case-Control Studies Cancer research biology.protein Female Antibody Signal transduction |
| Zdroj: | Genetic testing and molecular biomarkers. 21(2) |
| ISSN: | 1945-0257 |
| Popis: | Rheumatoid arthritis (RA) is characterized by the production of an array of proinflammatory cytokines through the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. Interleukin-1 receptor (IL-1R) and Toll-like receptors contain a common cytoplasmic motif the Toll/IL-1R (TIR) homology domain. This motif is required for NF-κB activation. IL-1R-associated kinase 1 (IRAK1) is a key adapter molecule recruited during the signaling cascade of the TIR. Its gene expression is regulated by the micro-RNA (miR)-146a.We investigated the role of IRAK1 single-nucleotide polymorphism (SNP) rs3027898 (IRAK1 rs3027898) and miR-146a SNP rs2910164 (miR-146a rs2910164) in Tunisian patients with RA and their association with C reactive protein (CRP), rheumatoid factor (RF), anticyclic citrullinated peptide (anti-CCP) antibodies, and erosion.In a cohort of 172 adult RA patients and 224 matched controls, IRAK1 rs3027898 genotyping was determined by mutagenically separated polymerase chain reaction (MS-PCR) with newly designed primers, and miR-146a rs2910164 genotyping was determined by fragment length polymorphism PCR-restriction (RFLP-PCR).The IRAK1 rs3027898 A allele was detected in 67% of RA patients and 70% of controls indicating that it is not associated with RA in codominant, dominant, or recessive models even after stratification by age and gender. The miR-146a rs2910164 G allele was detected in 76% of RA patients and 68% of controls, thus the C allele confers some protection based on a dominant model [CC+GC (odds ratio (95% confidence interval) = 0.6 (0.3-0.9), p = 0.03)]. No association with CRP, RF, anti-CCP, or erosion was found for either SNPs.The IRAK1 rs3027898 was not associated with RA, whereas C allele of miR-146a rs2910164 was found to be protective. Functional studies are required to investigate the exact role of miR-146a rs2910164 during RA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|