Disruption of the light cycle ablates diurnal rhythms in gastric vagal afferent mechanosensitivity
| Autor: | Andrew D. Vincent, Amanda J. Page, Stephen J. Kentish, Stewart Christie, Hui Li, Gary A. Wittert |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Physiology Photoperiod Stimulation Biology Diet High-Fat Satiety Response Fat pad 03 medical and health sciences Mice Random Allocation 0302 clinical medicine Rhythm Light Cycle Internal medicine Work Schedule Tolerance medicine Zeitgeber Animals Circadian rhythm Obesity Receptor Adiposity Afferent Pathways Endocrine and Autonomic Systems Body Weight Stomach Gastroenterology Shift Work Schedule Vagus Nerve Feeding Behavior Circadian Rhythm Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology Gastric Mucosa Energy Intake Mechanoreceptors 030217 neurology & neurosurgery Ex vivo |
| Zdroj: | Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility SocietyREFERENCES. 31(12) |
| ISSN: | 1365-2982 |
| Popis: | Background Gastric vagal afferents (GVAs) respond to mechanical stimulation, initiating satiety. These afferents exhibit diurnal fluctuations in mechanosensitivity, facilitating food intake during the dark phase in rodents. In humans, desynchrony of diurnal rhythms (eg, shift work) is associated with a higher risk of obesity. To test the hypothesis that shift work disrupts satiety signaling, the effect of a rotating light cycles on diurnal rhythms in GVA mechanosensitivity in lean and high-fat diet (HDF)-induced obese mice was determined. Methods Male C57BL/6 mice were fed standard laboratory diet (SLD) or HFD for 12 weeks. After 4 weeks, mice were randomly allocated to a normal light (NL; 12 hour light: 12 hour dark; lights on at zeitgeber time [ZT] 0) or rotating light (RL; 3-day NL cycle, 4-day reversed light cycle [lights on: ZT12] repeated) cycle for 8 weeks. At week 12, eight mice from each group were housed in metabolic cages. After 12 weeks, ex vivo GVA recordings were taken at 3 hour intervals starting at ZT0. Key results SLD-RL and HFD-RL gained more weight compared to SLD-NL and HFD-NL mice, respectively. Gonadal fat pad mass was higher in SLD-RL compared to SLD-NL mice. In SLD-NL mice, tension and mucosal receptor mechanosensitivity exhibited diurnal rhythms with a peak at ZT9. These rhythms were lost in SLD-RL, HFD-NL, and HFD-RL mice and associated with dampened diurnal rhythms in food intake. Conclusions & inferences GVA diurnal rhythms are susceptible to disturbances in the light cycle and/or the obese state. This may underpin the observed changes in feeding behavior. |
| Databáze: | OpenAIRE |
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