Extracellular High-Mobility Group Box 1 Acts as an Innate Immune Mediator to Enhance Autoimmune Progression and Diabetes Onset in NOD Mice
Autor: | Ya-fei Huang, Ping Yang, Mong Heng Wang, Jinxin Zhong, Feili Gong, Zheng Dong, Wenzhong Wei, Ying Wang, Jin-Xiong She, Sharad Purohit, Cong-Yi Wang, David M. Stern, Junyan Han |
---|---|
Rok vydání: | 2008 |
Předmět: |
Cell Survival
CD8 Antigens Endocrinology Diabetes and Metabolism Blotting Western Autoimmunity Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Nod CD8-Positive T-Lymphocytes Biology medicine.disease_cause Antibodies Cell Line Interferon-gamma Mice Immune system Mice Inbred NOD Insulin-Secreting Cells Internal Medicine medicine Animals HMGB1 Protein NOD mice Innate immune system Tumor Necrosis Factor-alpha Macrophages FOXP3 Forkhead Transcription Factors Dendritic Cells Dendritic cell Flow Cytometry medicine.disease Immunity Innate Recombinant Proteins CD11c Antigen Mice Inbred C57BL Diabetes Mellitus Type 1 Immunology Immunology and Transplantation Insulitis |
Zdroj: | Diabetes |
ISSN: | 1939-327X 0012-1797 |
Popis: | OBJECTIVE—The implication of innate immunity in type 1 diabetes development has long been proposed. High-mobility group box 1 (HMGB1), an evolutionarily conserved chromosomal protein, was recently recognized to be a potent innate inflammatory mediator when released extracellularly. We sought to test the hypothesis that HMGB1 acts as an innate immune mediator implicated in type 1 diabetes pathogenesis.RESEARCH DESIGN AND METHODS—Eight- and 12-week-old NOD mice were treated with an HMGB1 neutralizing antibody once a week until 25 weeks of age and monitored for insulitis progression and diabetes onset. The underlying mechanisms of HMGB1 regulation of autoimmune response were further explored.RESULTS—During autoimmunity, HMGB1 can be passively released from damaged pancreatic β-cells and actively secreted by islet infiltrated immune cells. Extracellular HMGB1 is potent in inducing NOD dendritic cell maturation and stimulating macrophage activation. Blockade of HMGB1 significantly inhibited insulitis progression and diabetes development in both 8- and 12-week-old NOD mice. HMGB1 antibody treatment decreased the number and maturation of pancreatic lymph node (PLN) CD11c++CD11b+ dendritic cells, a subset of dendritic cells probably associated with autoantigen presentation to naïve T-cells, but increased the number for PLN CD4+Foxp3+ regulatory T-cells. Blockade of HMGB1 also decreased splenic dendritic cell allo-stimulatory capability associated with increased tolergenic CD11c+CD8a+ dendritic cells. Interestingly, the number of CD8+interferon-γ+ (Tc1) T-cells was increased in the PLNs and spleen after blockade of HMGB1, which could be associated with retarded migration of activated autoreactive T-cells into the pancreatic islets.CONCLUSIONS—Extracellular HMGB1 functions as a potent innate immune mediator contributing to insulitis progression and diabetes onset. |
Databáze: | OpenAIRE |
Externí odkaz: |