Detection of Adult Green Sturgeon Using Environmental DNA Analysis
| Autor: | Paul S. Bergman, Scott M. Blankenship, Elizabeth Campbell, Gregg Schumer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Conservation Biology Endangered species lcsh:Medicine Artificial Gene Amplification and Extension Molecular biology assays and analysis techniques 01 natural sciences Polymerase Chain Reaction California Sturgeon Abundance (ecology) Environmental DNA lcsh:Science DNA extraction Conservation Science Multidisciplinary biology Nucleic acid analysis Ecology Fishes Distinct population segment Habitats Habitat Osteichthyes Research Design Vertebrates DNA analysis Research Article Sturgeons 010603 evolutionary biology Extraction techniques Rivers Surface Water Green sturgeon Animals Molecular Biology Techniques Molecular Biology Survey Research 010604 marine biology & hydrobiology Ecology and Environmental Sciences Endangered Species lcsh:R Organisms Biology and Life Sciences DNA Sequence Analysis DNA biology.organism_classification Fishery Research and analysis methods Survey Methods Threatened species Earth Sciences lcsh:Q Hydrology |
| Zdroj: | PLoS ONE, Vol 11, Iss 4, p e0153500 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Environmental DNA (eDNA) is an emerging sampling method that has been used successfully for detection of rare aquatic species. The Identification of sampling tools that are less stressful for target organisms has become increasingly important for rare and endangered species. A decline in abundance of the Southern Distinct Population Segment (DPS) of North American Green Sturgeon located in California’s Central Valley has led to its listing as Threatened under the Federal Endangered Species Act in 2006. While visual surveys of spawning Green Sturgeon in the Central Valley are effective at monitoring fish densities in concentrated pool habitats, results do not scale well to the watershed level, providing limited spatial and temporal context. Unlike most traditional survey methods, environmental DNA analysis provides a relatively quick, inexpensive tool that could efficiently monitor the presence and distribution of aquatic species. We positively identified Green Sturgeon DNA at two locations of known presence in the Sacramento River, proving that eDNA can be effective for monitoring the presence of adult sturgeon. While further study is needed to understand uncertainties of the sampling method, our study represents the first documented detection of Green Sturgeon eDNA, indicating that eDNA analysis could provide a new tool for monitoring Green Sturgeon distribution in the Central Valley, complimenting traditional on-going survey methods. |
| Databáze: | OpenAIRE |
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