Heat Shock Factor 1 Deficiency Affects Systemic Body Temperature Regulation
| Autor: | Hans Reinke, Charlotte von Gall, Horst-Werner Korf, Anna Stahr, Erik Noichl, Marc Ingenwerth |
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| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Tyrosine 3-Monooxygenase Endocrinology Diabetes and Metabolism Hypothalamus Thyrotropin Enzyme-Linked Immunosorbent Assay Eating Mice 03 medical and health sciences Cellular and Molecular Neuroscience Endocrinology Heat Shock Transcription Factors Heat shock protein Internal medicine Brown adipose tissue medicine Animals Heat shock HSF1 Mice Knockout Analysis of Variance Microscopy Confocal Endocrine and Autonomic Systems business.industry Prolactin receptor fungi Thermoregulation Prolactin DNA-Binding Proteins Heat shock factor 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation business Locomotion Body Temperature Regulation Transcription Factors |
| Zdroj: | Neuroendocrinology. 103:605-615 |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000441947 |
| Popis: | Introduction: Heat shock factor 1 (HSF1) is a ubiquitous heat-sensitive transcription factor that mediates heat shock protein transcription in response to cellular stress, such as increased temperature, in order to protect the organism against misfolded proteins. In this study, we analysed the effect of HSF1 deficiency on core body temperature regulation. Materials and Methods: Body temperature, locomotor activity, and food consumption of wild-type mice and HSF1-deficient mice were recorded. Prolactin and thyroid-stimulating hormone levels were measured by ELISA. Gene expression in brown adipose tissue was analysed by quantitative real-time PCR. Hypothalamic HSF1 and its co-localisation with tyrosine hydroxylase was analysed using confocal laser scanning microscopy. Results: HSF1-deficient mice showed an increase in core body temperature (hyperthermia), decreased overall locomotor activity, and decreased levels of prolactin in pituitary and blood plasma reminiscent of cold adaptation. HSF1 could be detected in various hypothalamic regions involved in temperature regulation, suggesting a potential role of HSF1 in hypothalamic thermoregulation. Moreover, HSF1 co-localises with tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis, suggesting a potential role of HSF1 in the hypothalamic control of prolactin release. In brown adipose tissue, levels of prolactin receptor and uncoupled protein 1 were increased in HSF1-deficient mice, consistent with an up-regulation of heat production. Conclusion: Our data suggest a role of HSF1 in systemic thermoregulation. |
| Databáze: | OpenAIRE |
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