Indomethacin and ibuprofen effect on IL-1ra production by mononuclear cells of preterm newborns and adults
| Autor: | I. Punsky, Hanna Bessler, M. Bergman, S Ziyada, Lea Sirota |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
medicine.medical_specialty Aging Lipopolysaccharide medicine.medical_treatment Sialoglycoproteins Indomethacin Ibuprofen Peripheral blood mononuclear cell Umbilical cord Monocytes chemistry.chemical_compound Immune system Internal medicine medicine Humans business.industry Anti-Inflammatory Agents Non-Steroidal Infant Newborn Interleukin Middle Aged Interleukin 1 Receptor Antagonist Protein Cytokine Endocrinology medicine.anatomical_structure chemistry Cord blood Pediatrics Perinatology and Child Health Toxicity Immunology business Infant Premature Developmental Biology |
| Zdroj: | Biology of the neonate. 82(2) |
| ISSN: | 0006-3126 |
| Popis: | The in vitro effect of indomethacin (IM) and ibuprofen (IB) on the production of the interleukin-1 receptor antagonist (IL-1ra) by cord blood mononuclear cells (CBMC) from preterm newborns was compared to that of peripheral blood mononuclear cells (PBMC) from adults. Mononuclear cells (MC) were incubated with lipopolysaccharide (LPS) in the absence or presence of various concentrations of IM and IB. The level of IL-1ra in the supernatants was tested by ELISA. The results showed a lower ability of MC from preterm newborns to produce IL-1ra as compared with adult cells, supporting the assumption of neonatal immune cell immaturity. IM at pharmacological concentrations caused inhibition of IL-1ra secretion by PBMC from adults whereas IB suppressed the secretion of IL-1ra at higher concentrations only. At the same concentrations neither drug had an in vitro effect on the production of IL-1ra by CBMC of preterm newborns. In conclusion, the lower ability of CBMC of preterm newborns to produce IL-1ra in response to LPS and the absence of an IM and IB effect on the secretion of this cytokine by these cells as compared with PBMC of adults, suggest an underdevelopment of the immune response in preterm newborns. |
| Databáze: | OpenAIRE |
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