The roles and mechanisms of homogalacturonan and rhamnogalacturonan I pectins on the inhibition of cell migration
Autor: | Siwen Yang, Yifa Zhou, Guihua Tai, Yuying Fan, Lin Sun, Congcong He |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
animal structures food.ingredient Pectin Galectin 3 Gene Expression Panax Lactose macromolecular substances 02 engineering and technology Polysaccharide complex mixtures Biochemistry Article Cell Line Focal adhesion Mice 03 medical and health sciences Ginseng food Cell Movement Structural Biology Cell Adhesion Animals Cell adhesion Cell Shape Molecular Biology Actin Cell Size chemistry.chemical_classification Focal Adhesions Chemistry digestive oral and skin physiology food and beverages Drug Synergism Cell migration General Medicine Fibroblasts 021001 nanoscience & nanotechnology Actin Cytoskeleton 030104 developmental biology Galectin-3 Biophysics Pectins 0210 nano-technology |
Zdroj: | Int J Biol Macromol |
ISSN: | 0141-8130 |
Popis: | Our previous paper reported the structure of ginseng pectic polysaccharides related to the cell migration inhibitory effects, but the underlying mechanisms are poorly understood. In this manuscript, rhamnogalacturonan I (RGI)-rich pectins prepared from ginseng pectin were investigated for their effect on cell migration. The results indicated that the combination of homogalacturonan (HG) and RGI-rich pectins exerted stronger effects than either HG- or RGI-rich pectin alone. Further studies revealed that the effects of HG- and RGI-rich pectins were dependent on pretreatment, which caused alterations in cell morphologies such as cell size and shape, focal adhesion, and the organization of actin filaments, suggesting that HG and RGI pectins exert synergistic effects on cell migration, likely through different ways. Morphological data and quantitative cell adhesion and spreading assays showed that HG- and RGI-rich pectin treatment decreased cell adhesion and cell spreading on the substratum, suggesting that HG- and RGI-rich pectins may exert their effects on cell migration via decreasing cell adhesion and cell spreading. Additionally, we showed that L-929 cells expressed little galectin-3 (Gal-3) and that lactose, an inhibitor of Gal-3 did not block the activities of HG- and RGI-rich pectins, implicating that cell migration inhibited by pectin did not correlate to Gal-3. |
Databáze: | OpenAIRE |
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