Globin mRNA Species Containing Poly(A) Segments of Different Lengths. Their Functional Stability in Xenopus Oocytes
| Autor: | Leclercq M, Uriel Z. Littauer, E. Hubert, Georges Huez, Uri Nudel, Hermona Soreq, Hubert Chantrenne, Gérard Marbaix |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Xenopus
Adenylate kinase medicine.disease_cause Biochemistry Drug Stability Functional stability Escherichia coli medicine Animals heterocyclic compounds RNA Messenger Polynucleotide phosphorylase Incubation Ovum Phosphorolysis Polyribonucleotide Nucleotidyltransferase Messenger RNA Base Sequence biology Temperature biology.organism_classification Molecular biology Globins Molecular Weight Kinetics Protein Biosynthesis Oocytes Female Rabbits Poly A |
| Zdroj: | European Journal of Biochemistry. 64:115-121 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1976.tb10279.x |
| Popis: | Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)-cellulose chromatography at 22 degrees C and at 4 degrees C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as poly(A)-free globin MRNA. |
| Databáze: | OpenAIRE |
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