Detection of human herpesvirus-6 in mesial temporal lobe epilepsy surgical brain resections
| Autor: | Riccardo Cassiani-Ingoni, William H. Theodore, P. Cogen, John D. Heiss, Nahid Akhyani, Susumu Sato, Steve Jacobson, Anna Fogdell-Hahn, Claudio Cermelli, Alexander O. Vortmeyer, D. Donati, William D. Gaillard |
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| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Herpesvirus 6 Human viruses Blotting Western Roseola Infantum Biology HHV-6 human herpesvirus 6 epilepsy neurology Article Temporal lobe Viral Proteins Epilepsy Glial Fibrillary Acidic Protein medicine Humans Child Antigens Viral Multiple sclerosis Limbic encephalitis Brain virus diseases Middle Aged biology.organism_classification medicine.disease Immunohistochemistry Temporal Lobe DNA-Binding Proteins medicine.anatomical_structure Epilepsy Temporal Lobe DNA Viral Neuroglia Female Human herpesvirus 6 Neurology (clinical) Encephalitis |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1526-632X 0028-3878 |
| DOI: | 10.1212/01.wnl.0000094357.10782.f9 |
| Popis: | The human herpesvirus-6 (HHV-6) is a ubiquitous β-herpesvirus,1,2 which infects a wide spectrum of cell types including glial cells.3–5 The virus is acquired early in childhood and is the causative agent of roseola infantum (exanthem subitum), a benign and otherwise self-limiting disease.6 Primary infection may result in seizures and severe neurologic complications such as meningitis or meningoenceph-alitis.7 As with all herpesviruses, HHV-6 can establish lifelong latent infection,8 and reactivation can occur in immunosuppressed patients such as bone marrow transplant recipients.9 Two HHV-6 variants have been characterized with respect to their antigenic and genomic composition.10 HHV-6B is primarily associated with most symptomatic infections during infancy,11 whereas HHV-6A has been suggested to be more neurotropic and is associated with viral persistence and reactivation in the CNS.12 HHV-6 variant A has also been detected more frequently in multiple sclerosis patient samples compared with variant B.13 However, HHV-6B has also been shown to have neurotropic potential4,14 and has recently been associated with limbic encephalitis.15 Although the role of HHV-6 in human CNS disease remains to be fully defined, a number of studies have suggested that the CNS can be a site for persistent HHV-6 infection.16,17 Autopsy material from a wide variety of normal18 and diseased19,20 brains has documented HHV-6 infection in the CNS, although its role as a causative agent is unclear. Cell types reported to be infected include oligodendrocytes, astrocytes, and possibly neurons.21,22 In limbic encephalitis, HHV-6 DNA was detected in the CSF, and astrocytes were shown to be the most represented cell type harboring HHV-6B in the hippocampus.15 This localization is consistent with the report of HHV-6 hippocampal encephalitis after bone marrow transplantation23 and of hippocampal injury in patients with prolonged focal febrile seizures,24,25 a frequent complication of a primary HHV-6 infection. In an attempt to characterize further the extent and distribution of HHV-6 in human glial cells, brain tissues from surgical specimens were processed to establish cultures of primary astrocytes and oligodendrocytes to be used for in vitro virologic examinations. Brain resections are a therapy for pharmacologically untreatable seizures,26 and specimens from patients with mesial temporal lobe epilepsy (MTLE) and neocortical epilepsy (NE) were collected for analysis. |
| Databáze: | OpenAIRE |
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