Impact of bacterial traces belonging to the Enterobacteriaceae on the prevalence of Chlamydia trachomatis in women visiting a community hospital in Japan
| Autor: | Takanori Watanabe, Keisuke Taki, Kiyotaka Abe, Kouhei Sakai, Mizue Matsushita, Kunihiro Minami, Torahiko Okubo, Junji Matsuo, Hiroyuki Yamaguchi |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Microbiology (medical) 030106 microbiology Chlamydia trachomatis Hospitals Community medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Enterobacteriaceae Japan RNA Ribosomal 16S Lactobacillus Prevalence medicine Humans Pharmacology (medical) Correlation of Data Phylogeny biology Microbiota Vaginosis Bacterial Bacteria Present Chlamydia Infections biology.organism_classification 16S ribosomal RNA medicine.disease Infectious Diseases chemistry Vagina Female Nugent score Bacterial vaginosis MacConkey agar |
| Zdroj: | Journal of Infection and Chemotherapy. 24:815-821 |
| ISSN: | 1341-321X |
| DOI: | 10.1016/j.jiac.2018.06.011 |
| Popis: | We explored the bacteria present in the vaginal microbiota facilitating the prevalence of Chlamydia trachomatis in women visiting a community hospital in Sapporo, Japan, by amplicon sequencing. A total of 273 cervical swab samples were collected, and bacterial vaginosis was evaluated in all specimens by assessment of the Nugent score. In 16 of the samples, bacterial 16S rDNA could not be detected and they were therefore omitted from subsequent experiments (n = 257). A significant negative correlation was observed between the Nugent scores and the amount of Lactobacillus 16S rDNA. Among the 257 samples, chlamydial plasmid was detected in 20 samples and was used for amplicon sequencing. No significant association between the Nugent score and the prevalence of C. trachomatis was detected. Based on the results of chlamydial plasmid detection and the Nugent score, chlamydia-negative samples (n = 27) were randomly selected. Finally, the number of operational taxonomic units (OTUs) obtained from amplicon sequencing was compared between chlamydia-positive (n = 20) and -negative samples (n = 27), revealing that a significant difference was only detected for the OTU numbers of Enterobacteriaceae between the C. trachomatis-positive and -negative groups. However, almost all of the samples utilized for amplicon sequencing failed to grow on MacConkey agar plates and produce indole. Taken together, we concluded that traces of bacteria, not live bacteria, belonging to the Enterobacteriaceae indicated the flow of bacteria through the anogenital route along with gut indole, and the resulting impact on the prevalence of C. trachomatis in the cervicogenital tract of women in Japan. |
| Databáze: | OpenAIRE |
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