Transient Receptor Potential Cation Channel Subfamily Vanilloid Member 3 is not Involved in Plasma Membrane Stretch-induced Intracellular Calcium Signaling in Merkel Cells
| Autor: | Masakazu Tazaki, Tadashi Furuya, Yoshiyuki Shibukawa, Maki Kimura, Toru Sato, Kunio Kobune, Yuki Kojima, Akinori Shinya, Asuka Higashikawa, Kazuhiro Ogura, Hiroyuki Mochizuki, Masaki Sato, Toshiyuki Sase |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
TRPV3 Cell Membrane TRPV Cation Channels General Medicine Biology Bioinformatics TRPV Calcium in biology Merkel Cells Cell biology Transient receptor potential channel chemistry Cricetinae Extracellular Animals Ankyrin Calcium Calcium Signaling Mechanotransduction Intracellular |
| Zdroj: | The Bulletin of Tokyo Dental College. 56:259-262 |
| ISSN: | 0040-8891 |
| DOI: | 10.2209/tdcpublication.56.259 |
| Popis: | Merkel cells (MCs), which form part of the MC-neurite complex, making contact with sensory afferents to drive mechanosensory transduction mechanisms, express transient receptor potential (TRP) cation channel subfamily vanilloid (V) members 1, 2, and 4, as well as ankyrin subfamily member 1. While these proteins are involved in sensing plasma membrane stretch, less is known about the functional properties of TRPV subfamily member 3 (TRPV3) during membrane stretch in MCs. The aim of this study was to determine whether TRPV3 channels were involved in mechanosensory activity by measuring intracellular free Ca(2+) concentrations ([Ca(2+)]i) in MCs isolated from hamster buccal mucosa. Application of a hypotonic extracellular solution to quinacrine-positive MCs elicited a transient increase in [Ca(2+)]i. When TRPV3 channel antagonist 2,2-diphenyltetrahydrofuran was added to the hypotonic extracellular solution, however, no effect was observed on hypotonic stimulation-induced increase in [Ca(2+)]i. These results suggest that TRPV3 channels are not involved in the mechanosensory mechanism during membrane stretch in MCs. |
| Databáze: | OpenAIRE |
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