Nitrogen oxide air pollutants interfere with the measurement of nitric oxide using 2,3-diaminonaphthalene: Reduction of background interference

Autor:	Yumi Hatori, Yang-Il Fang, Kazuo Honda, Hisayuki Ohata
Rok vydání:	2009
Předmět:	Air Pollutants Chromatography Metabolite Biophysics Endothelial Cells Cell Biology Nitric Oxide Biochemistry Nitric oxide chemistry.chemical_compound chemistry Air pollutants 2-Naphthylamine Environmental chemistry Nitrogen oxide Nitrite No production Molecular Biology Incubation Cells Cultured
Zdroj:	Analytical Biochemistry. 393:132-134
ISSN:	0003-2697
DOI:	10.1016/j.ab.2009.06.023
Popis:	Nitrite, a stable metabolite of nitric oxide (NO), is measured fluorometrically as an indicator of NO production using 2,3-diaminonaphthalene. In cultured cells, it has been believed that a longer period of incubation improves the detection sensitivity because of the accumulation of nitrite formed from NO in culture media. However, here we show that nitrite formed from nitrogen oxide air pollutants accumulates continuously in culture media during the incubation and interferes with the measurement of NO as nitrite. Thus, a proper period of incubation is important to allow maximum nitrite signals from NO with minimum background nitrite from the air.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6fab322f7c6354f07ab3820c28a7c6b https://doi.org/10.1016/j.ab.2009.06.023 Zobrazit plný text záznamu Full Text from ScienceDirect