Surfactant Protein A Suppresses Lipopolysaccharide-Induced IL-10 Production by Murine Macrophages
| Autor: | Laurent Salez, Nico van Rooijen, Michel Chignard, Viviane Balloy, Mai Lebastard, Francis X. McCormack, Lhousseine Touqui |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Male Pulmonary Surfactant-Associated Proteins Lipopolysaccharide Proteolipids Immunology Bone Marrow Cells Inflammation Cell Separation Biology Monocytes Mice chemistry.chemical_compound Cell Movement In vivo Macrophages Alveolar medicine Animals Humans Immunology and Allergy Lung Pulmonary Surfactants In vitro Interleukin-10 Cell biology Surfactant protein A Mice Inbred C57BL Immunosurveillance Interleukin 10 medicine.anatomical_structure chemistry medicine.symptom Bronchoalveolar Lavage Fluid Immunosuppressive Agents |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.166.10.6376 |
| Popis: | Upon LPS exposure, mononuclear phagocytes produce TNF-α and IL-10, two cytokines with pro- and anti-inflammatory activities, respectively. We previously described that murine resident alveolar macrophages, which play a central role in the immunosurveillance of the lung alveoli, do not synthesize IL-10 in vivo or in vitro when exposed to LPS. In the present report we demonstrate that during lung inflammation induced by the intranasal administration of LPS, bronchoalveolar cells collected between days 3 and 5 are able to synthesize IL-10 when exposed to LPS. We also show that depletion of resident alveolar macrophages by an intratracheal instillation of liposome-encapsulated clodronate is followed by subsequent replenishment of the airspaces by mononuclear phagocytes. This is accompanied by the transient competence of cells for IL-10 production. The cell capacity to produce IL-10 is evident up to 3 days and then decreases. This led us to hypothesize that the alveolar environment contains a down-regulator of LPS-induced IL-10 synthesis by recently emigrating mononuclear phagocytes. We show that the surfactant protein A, an airspace protein that has known immunomodulatory activities, dramatically inhibits LPS-induced IL-10 formation by bone marrow-derived macrophages. These data show a difference between resident and inflammatory macrophages with respect to IL-10 synthesis. Moreover, this study highlights for the first time the inhibitory role of surfactant protein A in the anti-inflammatory activity of macrophages through inhibition of IL-10 production. |
| Databáze: | OpenAIRE |
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