Fear conditioning is associated with altered integration of PLC and ERK signaling in the hippocampus
| Autor: | Kevin K. Caldwell, Colin T Buckley |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
MAPK/ERK pathway
MAP Kinase Signaling System Clinical Biochemistry Phospholipase C beta Biology Phospholipase C gamma Hippocampal formation Toxicology Hippocampus Biochemistry Isozyme Rats Sprague-Dawley Serine Behavioral Neuroscience Conditioning Psychological Animals Extracellular Signal-Regulated MAP Kinases Biological Psychiatry Mitogen-Activated Protein Kinase 1 Pharmacology Mitogen-Activated Protein Kinase 3 Phospholipase C Kinase Fear Rats Cell biology Isoenzymes Type C Phospholipases Synaptic plasticity Female Neuroscience hormones hormone substitutes and hormone antagonists |
| Zdroj: | Pharmacology Biochemistry and Behavior. 79:633-640 |
| ISSN: | 0091-3057 |
| DOI: | 10.1016/j.pbb.2004.09.013 |
| Popis: | The extracellular signal-regulated protein kinases (ERKs) are proline-directed, serine/threonine kinases that regulate a variety of cellular functions, including proliferation, differentiation, and plasticity. In the present report, we provide evidence that ERK2 and phosphatidylinositol-specific phospholipase C (PLC)-beta and -gamma isozymes interact in the rat hippocampal formation. We found that anti-PLC-beta1a, -beta2, -beta4, -gamma1 and -gamma2, but not -beta3, immune complexes isolated from rat hippocampal formation postnuclear fractions contain anti-ERK2 immunoreactivity. Further, we show that PLC catalytic activity is associated with anti-ERK2 immunoprecipitates isolated from the hippocampal formation, and that the amount of enzyme activity is significantly increased following fear-conditioned learning. The observed interactions may be mediated by consensus sequences conforming to an ERK2 docking site, termed a D-domain, that we identified in PLC-beta1a, -beta2, -beta4 -gamma1 and -gamma2. Based on these results, we propose that PLC-beta and PLC-gamma isozymes form signaling complexes with ERK2 in rat brain, and these complexes play critical roles in learning and memory, as well as a variety of other neuronal functions. |
| Databáze: | OpenAIRE |
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