Evidences for the existence of a low dopaminergic tone in polycystic ovarian syndrome: implications for OHSS development and treatment

Autor: Francisco Gaytan, Hortensia Ferrero, Raúl Gómez, Francisco Delgado-Rosas, Carlos Simón, María Gaytán, Antonio Pellicer, Concepción Morales, Ralf C. Zimmermann
Rok vydání: 2011
Předmět:
Adult
Vascular Endothelial Growth Factor A
endocrine system
medicine.medical_specialty
Cabergoline
endocrine system diseases
Angiogenesis
Endocrinology
Diabetes and Metabolism
Dopamine
Clinical Biochemistry
Ovarian hyperstimulation syndrome
Neovascularization
Physiologic
Context (language use)
Ovary
Tissue Banks
Biology
Biochemistry
chemistry.chemical_compound
Ovarian Hyperstimulation Syndrome
Young Adult
Endocrinology
Risk Factors
Internal medicine
Luteal Cells
medicine
Humans
Ovarian follicle
Ergolines
Cells
Cultured
Granulosa Cells
Receptors
Dopamine D2
Biochemistry (medical)
Kinase insert domain receptor
medicine.disease
Vascular Endothelial Growth Factor Receptor-2
female genital diseases and pregnancy complications
Vascular endothelial growth factor
medicine.anatomical_structure
chemistry
Dopamine Agonists
Oocytes
Female
medicine.drug
Polycystic Ovary Syndrome
Signal Transduction
Zdroj: The Journal of clinical endocrinology and metabolism. 96(8)
ISSN: 1945-7197
Popis: The dopamine/dopamine receptor 2 (D2/Drd2) pathway modulates vascular endothelial growth factor (VEGF)-dependent vascular permeability and angiogenesis in the ovary. Deregulation of the VEGF/VEGF receptor (VEGFR)-2 pathway leading to increased risk of ovarian hyperstimulation syndrome has been described in the ovary of patients suffering from polycystic ovarian syndrome (PCOS).The objective of the study was to ascertain whether deregulation of the VEGF/VEGFR-2 might a least be partially due to abnormalities of the D2/Drd2 pathway in PCOS women.Dated, archived ovaries from PCOs and control group patients as well as human chorionic gonadotropin-stimulated luteinized granulosa cells form PCOS and non-PCOS oocyte patients were used.The study was conducted at a private research center.PCOS and nonpolycystic ovarian patients and oocyte patients participated in the study.Human ovarian sections were stained against the Drd2 antibody. Human chorionic gonadotropin-stimulated luteinized granulosa cells (LGC) were cultured in the presence/absence and the Drd2 agonist cabergoline.Drd2 and vascularized stained area in the theca layer of antral (8 mm) and luteinized follicles was quantified. VEGF, D2, and its related metabolites were measured in the supernatant of cultured LGC by ELISA and HPLC, respectively. VEGFR-2 and Drd2 expressed by LGC was quantified through an In-Cell ELISA.Decreased Drd2 expression and increased vascularization in the theca layer of antral and luteinized follicles of PCOS ovaries was observed. A lower dopamine production and reduced efficacy of cabergoline in inhibiting VEGF secretion was uncovered in LGC from PCOS.Decreased dopaminergic tone as well as deregulated Drd2 signaling might explain higher VEGF and vascularization leading to increased ovarian hyperstimulation syndrome risk in PCOS.
Databáze: OpenAIRE
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