Immunization of mice with irradiated Trypanosoma cruzi grown in cell culture: Relation of numbers of parasites, immunizing injections, and route of immunization to resistance
| Autor: | Willie L. Chapman, Virginia B. Waits, William L. Hanson |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Chagas disease
Trypanosoma Trypanosoma cruzi Parasitemia Mice Subcutaneous injection medicine Animals Chagas Disease Cells Cultured Infectivity Mice Inbred C3H biology medicine.disease biology.organism_classification Virology Radiation Effects Blood Infectious Diseases Immunization Cell culture Vero cell Female Parasitology |
| Zdroj: | International Journal for Parasitology. 6:341-347 |
| ISSN: | 0020-7519 |
| Popis: | Mice were given 5 or 8 weekly injectins of either 2·0 × 10 6 or 20·0 × 10 6 irradiated T. cruzi from cell culture (ratio of trypomastigotes to amastigotes, 1 : 1) via the intraperitoneal route or via the subcutaneous route and challenged via the subcutaneous route one week after the last injection with 5·0 × 10 4 T. cruzi in mouse blood. The irradiated parasites used were not capable of producing infections in either Vero cell cultures or C 3 H mice. Mice receiving irradiated parasites were significantly protected against the challenge infection as evidenced by significantly lower mean parasitemia, lessened signs of acute disease, and reduced mortality than that observed in untreated controls. Mice receiving 5 weekly immunizing injections of irradiated parasites were more resistant to challenge than those receiving 3 in previous work. Mice receiving 8 weekly immunizing injections were not significantly more protected against challenge than those receiving 5. Mice given 5 weekly injections of 20·0 × 10 6 irradiated parasites were significantly more resistant to challenge than those receiving 2·0 × 10 6 irradiated parasites on the same schedule. Mice given 5 weekly intraperitoneal injections of 20·0 × 10 6 irradiated parasites were significantly more resistant to challenge than those receiving an equivalent number of immunizing injections via the subcutaneous route. |
| Databáze: | OpenAIRE |
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