Lipopolysaccharide increases lysozyme adhesion to chitin
| Autor: | I. A. Selivanskaia, L. M. Mudrik, A. P. Levitsky, T. A. Sevostianova, L. N. Khromagina |
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| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Chromatography Lipopolysaccharide Chemistry fungi Substrate (chemistry) macromolecular substances Adhesion chitin Education carbohydrates (lipids) chemistry.chemical_compound Adsorption Enzyme bacteriolysis Chitin lysozyme lipopolysaccharide GV557-1198.995 Medicine Lysozyme adsorption of lysozyme on chitin Sports Egg white |
| Zdroj: | Journal of Education, Health and Sport, Vol 9, Iss 11, Pp 240-247 (2019) |
| ISSN: | 2391-8306 |
| DOI: | 10.5281/zenodo.3558920 |
| Popis: | Levitsky A. P., Mudrik L. M., Khromagina L. N., Sevostianova T. A., Selivanskaia I. A. Lipopolysaccharide increases lysozyme adhesion to chitin. Journal of Education, Health and Sport. 2019;9(11):240-247. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.3558921 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2019.09.11.021 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2019; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 06.11.2019. Revised: 14.11.2019. Accepted: 29.11.2019. UDC 616.153:577.152:616.633:612.31 LIPOPOLYSACCHARIDE INCREASES LYSOZYME ADHESION TO CHITIN A. P. Levitsky, L. M. Mudrik, L. N. Khromagina, T. A. Sevostianova, I. A. Selivanskaia State Etablishment «The Institute of Stomatology and Maxillo-Facial Surgery of the National Academy of Medical Science of Ukraine» flavan.ua@gmail.com Abstract Background. To study the effect of lipopolysaccharide on the adsorption of lysozyme on chitin. Methods. Egg white lysozyme, lipopolysaccharide and chitin were used. The lyophilized cells of M. lyzodeikticus were used as a lysozyme substrate. Lysozyme activity was determined by the bacteriolytic method using different concentrations (8-512 μg/ml). Lysozyme adsorption on chitin was determined by the loss of lysozyme from solution after shaking with chitin. The effect of lipopolysaccharide (0.6-40 μg/ml) on lysozyme adsorption was evaluated by the loss of lysozyme from the solution after shaking with 50 mg of chitin. Results. In determining the activity of lysozyme by the bacteriolytic method, the linear nature of the dependence of activity on the concentration of the enzyme is observed only at low concentrations (8-100 μg / ml). Lipopolysaccharide does not affect the bacteriolytic activity of lysozyme, however, dose-dependently increases the adsorption of lysozyme on chitin. Conclusion. Lipopolysaccharide increases the adsorption of lysozyme on chitin. Keywords: lysozyme, lipopolysaccharide; chitin; bacteriolysis; adsorption of lysozyme on chitin. |
| Databáze: | OpenAIRE |
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