SufC may promote the survival of Salmonella enterica serovar Typhi in macrophages
| Autor: | Min Wang, Lin Qi, Yan Xiao, Chenhao Qin, Haifang Zhang, Yongmei Sheng, Hong Du |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Mutant
Oxidative phosphorylation Biology medicine.disease_cause Microbiology Bacterial Proteins medicine Humans Typhoid Fever Escherichia coli Gene Adenosine Triphosphatases Microbial Viability Interleukin-6 Tumor Necrosis Factor-alpha Macrophages Gene Expression Regulation Bacterial Salmonella typhi biology.organism_classification Oxidative Stress Infectious Diseases Salmonella enterica serovar Typhi Salmonella enterica Biogenesis Oxidative stress |
| Zdroj: | Microbial Pathogenesis. 85:40-43 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2015.05.006 |
| Popis: | The sufC gene of Escherichia coli (E. coli) is required for the biogenesis of iron-sulfur (Fe-S) cluster under oxidative stress conditions. In order to investigate the roles of sufC in Salmonella enterica serovar Typhi (S. Typhi), isogenic S. Typhi strain GIFU10007 harboring a non-polar mutation of sufC (ΔsufC) was constructed and the results showed that the sufC deleted mutant grew more slowly than the wild type strain when encounter oxidative stresses. Moreover, the deletion of sufC gene decreased S. Typhi survival within macrophages. After macrophages infected by sufC deleted mutant and wild type strain, we detected IL-6 and TNF-α released into the supernatant, and found the expression of IL-6 and TNF-α decreased in the supernatant of sufC deleted mutant infected groups than the wild type strain infected ones. In summary, our results showed that SufC may promote S. Typhi coping oxidative stress and help S. Typhi survival in macrophages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect