Heterologous expression of pentalenene synthase (PSS) from Streptomyces UC5319 in Xanthophyllomyces dendrorhous

Autor: Oliver Kayser, Elena Melillo, Remco Muntendam, Wim J. Quax
Přispěvatelé: Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Nanotechnology and Biophysics in Medicine (NANOBIOMED)
Rok vydání: 2012
Předmět:
Mutant
Colony Count
Microbial
Gene Expression
Applied Microbiology and Biotechnology
ARTEMISININ
SACCHAROMYCES-CEREVISIAE
chemistry.chemical_compound
Polyisoprenyl Phosphates
PHAFFIA-RHODOZYMA
Intramolecular Lyases
biology
HIGH-LEVEL PRODUCTION
Pentalenene synthase
General Medicine
Streptomyces
Phenotype
Biochemistry
ESCHERICHIA-COLI
11-DIENE
Sesquiterpenes
Biotechnology
Heterologous
AMORPHA-4
Bioengineering
Cyclopentanes
Gas Chromatography-Mass Spectrometry
CLONING
Transformation
Genetic
Astaxanthin
Escherichia coli
AMORPHA-4
11-DIENE
Terpenes
Basidiomycota
BETA-CAROTENE
biology.organism_classification
BIOSYNTHETIC-PATHWAY
GENE
Carotenoids
Yeast
Biosynthetic Pathways
Transformation (genetics)
chemistry
Genes
Bacterial
Mutation
biology.protein
Biocatalysis
Xanthophyllomyces
Heterologous expression
Zdroj: Journal of Biotechnology, 161(3), 302-307. Elsevier
ISSN: 1873-4863
0168-1656
Popis: For the first time, the pentalenene synthase (PSS) gene from Streptomyces UC5319 was expressed in Xanthophyllomyces dendrorhous, a native producer of astaxanthin. For the expression of the gene and the concurrent knock out of the native crtE or crtYB genes, two new vectors were engineered and used for the transformation of the wild-type strain of X. dendrorhous. The transformations resulted in white colonies, showing a complete shutdown of the carotenoid production. Furthermore, an additional vector was constructed for the insertion of the PSS gene in the rDNA of the yeast. All the mutant strains produce the sesquiterpene pentalenene and show no difference in growth when compared to the wild-type strain. In this report, we demonstrate that X. dendrorhous is a suitable host for the expression of heterologous terpene cyclases and for the production of foreign terpene compounds. (C) 2012 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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